Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4943 |
| PDBID: | 7STF |
| Chains: | HL_A |
| Organism: | Homo sapiens, synthetic construct |
| Method: | EM |
| Resolution (Å): | 3.14 |
| Reference: | 10.1038/s41467-023-40821-w |
| Antibody | |
| Antibody: | V2 IgG Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human HLA class I histocompatibility antigen, A alpha chain |
| Antigen mutation: | No |
| Durg Target: | P04439 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7STF_H|Chain A[auth H]|IgG, Fab Heavy Chain V2|Homo sapiens (9606) EVQLVESGGGLVQPGGSLRLSCAASGFNLSYSDIHWVRQAPGKGLEWVAVVMPDSGHTNYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCSRATNIPVYAFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEP |
Light Chain: L
Mutation: NULL
| >7STF_L|Chain C[auth L]|IgG, Fab Light Chain V2|Homo sapiens (9606) DIQMTQSPSSLSASVGDRVTITCRASQDVNTAVAWYQQKPGKAPKLLIYSASFLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQSYYYFRPITFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: NULL
| >7STF_A|Chain D[auth A]|HLA class I histocompatibility antigen, A alpha chain|Homo sapiens (9606) GSHSMRYFFTSVSRPGRGEPRFIAVGYVDDTQFVRFDSDAASQRMEPRAPWIEQEGPEYWDQETRNVKAQSQTDRVDLGTLRGYYNQSEAGSHTIQIMYGCDVGSDGRFLRGYRQDAYDGKDYIALNEDLRSWTAADMAAQITKRKWEAAHEAEQLRAYLDGTCVEWLRRYLENGKETLQRTDPPKTHMTHHPISDHEATLRCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDGTFQKWAAVVVPSGEEQRYTCHVQHEGLPKPLTLRWELSSQP |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition H: VAL2 PHE27 ASN28 LEU29 SER30 TYR31 SER32 VAL51 ASP54 SER55 GLY56 HIS57 TYR60 ASN101 ILE102 PRO103 TYR105 L: ASN30 THR31 ALA32 SER52 PHE53 ARG66 SER91 TYR92 TYR93 TYR94 ARG96 A: GLU19 PRO20 PHE36 SER38 GLN43 ASP61 ARG65 ASN66 LYS68 ALA69 GLN72 THR73 ARG75 VAL76 GLY79 THR80 LEU81 ARG82 GLY83 TYR84 ASN86 GLN87 GLU89 TYR123 ALA139 ILE142 THR143 ARG145 LYS146 ALA149 ALA150 HIS151 GLU152 GLN155 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
