Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4942 |
| PDBID: | 7ST8 |
| Chains: | HL_S |
| Organism: | Mus musculus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.75 |
| Reference: | 10.1107/S2059798322003011 |
| Antibody | |
| Antibody: | 7H2.2 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Metalloproteinase sperm acrosomal SLLP1 binding protein (SAS1B) C-terminal region |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7ST8_H|Chain A[auth H]|7H2.2 Fab Heavy Chain|Mus musculus (10090) QVTLKESGPGILQPSQTLSLTCSFSGFSLSTSGMGVGWIRQPSGKGLEWLAHIWWDDVKRYNPALKSRLTISKDTSSSQVFLKIASGDTIDTATYYCARIPTDDYYALDHWGQGASVTVSSAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVPRDC |
Light Chain: L
Mutation: NULL
| >7ST8_L|Chain B[auth L]|7H2.2 Fab Light Chain|Mus musculus (10090) DIVLTQSPASLAVSLGQRATISCRASKSVSTSGYSFMHWYQQKPGQPPKLLIYLASNLESGVPARFSGSGSGTDFTLNIHPVEEEDAATFYCQHSRELPYTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNE |
Antigen
Chain: S
Mutation: NULL
| >7ST8_S|Chain C[auth S]|Astacin-like metalloendopeptidase|Homo sapiens (9606) MGSSHHHHHHSSGLVPRGSHMASGPRPRGRGSHAHSTGRSPAPASLSLQRLLEALSAESRSPDPSGSSAGGQPVPAGPGESPHGWESPALKKLSAEASARQPQTLASSPRSRPGAGAPGVAQEQSWLAGVSTKPTVPSSEAGIQPVPVQGSPALPGGCVPRNHFKGMSED |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition H: TRP52 TRP53 ASP54 VAL56 ARG58 PRO61 ASP98 ASP99 L: THR27 SER28 TYR30 PHE32 SER91 ARG92 GLU93 LEU94 TYR96 S: ALA305 SER306 LEU307 SER308 LEU309 GLN310 ARG311 LEU313 GLU314 ALA315 SER317 ALA318 GLU319 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
