Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4935 |
| PDBID: | 7SRS |
| Chains: | QP_R |
| Organism: | Mus musculus, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.30 |
| Reference: | 10.1016/j.neuron.2022.08.006 |
| Antibody | |
| Antibody: | Anti-5HT2BR Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human 5-hydroxytryptamine receptor 2B |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: Q
Mutation: NULL
| >7SRS_Q|Chain B[auth Q]|Anti-5HT2BR Fab heavy chain|Mus musculus (10090) EVQLQQSGPELVKPGASVKLSCKASGYTFTSSWMHWVKQRPGQGLEWIGNIYPSNGGTNYNERFKSKATLTVDRSSNTAYMQLSSLTSEDSAVYFCARFGSFITTILTTYYNPVDYWGQGTTLTVSSASTKGPSVFPLAPSTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPASSLGTQTYICNVNHKPSNTKVDKKV |
Light Chain: P
Mutation: NULL
| >7SRS_P|Chain A[auth P]|Anti-5HT2BR Fab light chain|Mus musculus (10090) DIVLIQSPAIMSASPGEKVTITCSASSSVSYMHWFQQKPGTSPKLWIYSTSNLASGVPARFSGSGSGTSYSLTISRMEAEDAATYYCQQRSSYPLTFGAGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLPVTKSFNRG |
Antigen
Chain: R
Mutation: NULL
| >7SRS_R|Chain C[auth R]|5-hydroxytryptamine receptor 2B|Homo sapiens (9606) TESIPEEMKQIVEEQGNKLHWAALLILMVIIPTIGGNTLVILAVSLEKKLQYATNYFLMSLAVADLLVGLFVMPIALLTIMFEAMWPLPLVLCPAWLFLDVLFSTASIWHLCAISVDRYIAIKKPIQANQYNSRATAFIKITVVWLISIGIAIPVPIKGIETDVDNPNNITCVLTKERFGDFMLFGSLAAFFTPLAIMIVTYFLTIHALQKVRLLSGSRQTISNLQRASKVLGIVFFLFLLMWCPFFITNITLVLCDSCNQTTLQMLLEIFVWIGYVSSGVNPLVYTLFNKTFRDAFGRYITCNYRATKSVKTPMRLRSSTIQSSSIILLDTL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| P: SER30 TYR31 ARG90 SER91 SER92 TYR93 LEU95 Q: THR30 SER31 TRP33 HIS35 ASN50 TYR52 SER54 ASN55 GLY100 SER101 PHE102 ILE103 THR104 THR105 ILE106 LEU107 THR108 THR109 R: LEU125 VAL126 PRO129 ALA130 PRO191 ILE192 GLY194 ILE195 GLU196 THR197 ASP198 VAL199 ASP200 ASN201 ASN204 THR210 GLU212 ARG213 GLN355 THR356 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
