Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 489 |
| PDBID: | 3FMG |
| Chains: | HL_A |
| Organism: | Mus musculus, Simian rotavirus |
| Method: | XRD |
| Resolution (Å): | 3.40 |
| Reference: | 10.1126/science.1170481 |
| Antibody | |
| Antibody: | 4FB Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Glycoprotein VP7 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >3FMG_H|Chain B[auth H]|Fab of neutralizing antibody 4F8, heavy chain|Mus musculus (10090) QVQLQQSGAELMKPGALVKISCKATGYTFSSYWIEWVKQRPGHGLEWIGEILPGSGSINYNEKFKGKATFTADTSSNTAYMQLNSLTSEDSAVYYCARNHGSPDFHVMDYWGQGTSITVSSAKTTAPPVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSPRPSETVTCNVAHPASSTKVDKKIVPR |
Light Chain: L
Mutation: NULL
| >3FMG_L|Chain A[auth L]|Fab of neutralizing antibody 4F8, light chain|Mus musculus (10090) DIQMTQSPASLSASVGETVTITCRPSENIYSYLAWYQQKQGKSPQLLVYKAKTLPEGVPSRFSGSGSGTHFSLKISSVQPEDFGTYYCQHHNGPPLTFGAGTKLELKRADAAPTVSIFPPSSKQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNR |
Antigen
Chain: A
Mutation: NULL
| >3FMG_A|Chain C[auth A]|Glycoprotein VP7|Simian rotavirus (10922) QNYGINLPITGSMDTAYANSTQEETFLTSTLCLYYPTEAATEINDNSWKDTLSQLFLTKGWPTGSVYFKEYTDIASFSVDPQLYCDYNVVLMKYDATLQLDMSELADLILNEWLCNPMDITLYYYQQTDEANKWISMGSSCTIKVCPLNTQTLGIGCLTTDTATFEEVATAEKLVITDVVDGVNHKLDVTTATCTIRNCKKLGPRENVAVIQVGGSDVLDITADPTTAPQTERMMRINWKKWWQVFYTVVDYVNQIIQAMSKRSRSLNSAAFYYRI |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: PRO94 PRO95 H: SER56 GLY57 SER58 ILE59 ASN60 TYR61 GLU63 LYS66 A: LYS194 LEU208 THR210 ASP211 THR212 ALA213 THR214 PHE215 GLU217 THR220 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
