Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4883 |
| PDBID: | 7SGF |
| Chains: | MN_C |
| Organism: | Mammarenavirus lassaense, Oryctolagus cuniculus |
| Method: | EM |
| Resolution (Å): | 4.41 |
| Reference: | 10.1016/j.chom.2022.10.018 |
| Antibody | |
| Antibody: | LAVA01 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | GPC-I53-50A |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: M
Mutation: NULL
| >7SGF_M|Chain C[auth H], G[auth J], K[auth M]|LAVA01 mAb - Heavy Chain|Oryctolagus cuniculus (9986) QLLSCIALSLALVTNSQLEESGGGLVQTEGSLTLTCTASGFSFSNNDYICWVRQAPGKGLEWIGCIYSDYGFSFFATWAKDRFSGSKTSSTTVTLQGTGLTAADTATYFCVKTYVSRFGYYIRWDYFDLWGPGTLVIVSSGQ |
Light Chain: N
Mutation: NULL
| >7SGF_N|Chain D[auth K], H[auth L], L[auth N]|LAVA01 mAb - Light Chain|Oryctolagus cuniculus (9986) NSDQVMTQTPSPVSAAVGGTVSISCQSSKSVHNENFLSWYQQKPGQRPKLLIYRASTLASGVPSRFKGSGSGTQFTLTISDVQCDDAATYYCAGGDIQSSDDVFGGGTEVVVQGDPVAPS |
Antigen
Chain: C
Mutation: NULL
| >7SGF_C|Chain A, B, E[auth C], F[auth a], I[auth b], J[auth c]|GPC-I53-50A|Lassa mammarenavirus (3052310) MGQIVTFFQEVPHVIEEVMNIVLIALSVLAVLKGLYNFATCGLVGLVTFLLLCGRSCTTSLYKGVYELQTLELNMETLNMTMPLSCTKNNSHHYIMVGNETGLELTLTNTSIINHKFCNLSDAHKKNLYDHALMSIISTFHLSIPNFNQYEAMSCDFNGGKISVQYNLSHSYAGDAANHCGTVANGVLQTFMRMAWGGSYIALDSGCGNWDCIMTSYQYLIIQNTTWEDHCQFSRPSPIGYLGLLSQRTRDIYISRRRRGTFTWTLSDSEGKDTPGGYCLTRWMLIEAELKCFGNTAVAKCNEKHDEEFCDMLRLFDFNKQAIQRLKAPAQMSIQLINKAVNALINDQLIMKNHLRDIMCIPYCNYSKYWYLNHTTTGRTSLPKCWLVSNGSYLNETHFSDDIEQQADNMITEMLQKEYMERQGGSGGSGGSGGSGGSEKAAKAEEAARKMEELFKKHKIVAVLRANSVEEAIEKAVAVFAGGVHLIEITFTVPDADTVIKALSVLKEKGAIIGAGTVTSVEQCRKAVESGAEFIVSPHLDEEISQFCKEKGVFYMPGVMTPTELVKAMKLGHDILKLFPGEVVGPEFVKAMKGPFPNVKFVPTGGVDLDNVCEWFDAGVLAVGVGDALVEGDPDEVREKAKEFVEKIRGCTEGSLEWSHPQFEK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition M: ARG103 PHE104 GLY105 TYR106 TYR107 N: SER28 VAL29 HIS30 ASN31 GLU32 ASN33 C: ASN90 TYR200 ILE201 ALA202 LEU203 ASP204 SER205 GLY206 CYS207 SER216 TYR217 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
