Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4880 |
| PDBID: | 7SFW |
| Chains: | ST_K |
| Organism: | Venezuelan equine encephalitis virus (strain TC-83), Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.20 |
| Reference: | 10.1084/jem.20212532 |
| Antibody | |
| Antibody: | hVEEV-63 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | VEEV TC-83 virus-like particles spike glycoprotein E2 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: S
Mutation: NULL
| >7SFW_S|Chain M, O, Q, S|hVEEV-63 Fab heavy chain|Homo sapiens (9606) QVQLVQSGAEVKKPGASVKVSCKSSGYTFTNYIIHWVRQAPGQRLEWMGWINAGNGNTKYSQKFQGRISVTRDTSASAAYMELSSLKSEDTALYYCATLQMDYGGNGDLDYWGQGTLVTVSS |
Light Chain: T
Mutation: NULL
| >7SFW_T|Chain N, P, R, T|hVEEV-63 Fab light chain|Homo sapiens (9606) YELTQPPSVSVSPGQTARITCSGDALPKQYVYWYQQKPGQAPVLVIYKDSERPSGIPERFSGSSSGTTVTLTISGVQAEDDADYYCQAADSSNTEYVFGTGTKVTVL |
Antigen
Chain: K
Mutation: NULL
| >7SFW_K|Chain B, E, H, K|Spike glycoprotein E2|Venezuelan equine encephalitis virus (strain TC-83) (11037) STEELFNEYKLTRPYMARCIRCAVGSCHSPIAIEAVKSDGHDGYVRLQTSSQYGLDSSGNLKGRTMRYDMHGTIKEIPLHQVSLYTSRPCHIVDGHGYFLLARCPAGDSITMEFKKDSVRHSCSVPYEVKFNPVGRELYTHPPEHGVEQACQVYAHDAQNRGAYVEMHLPGSEVDSSLVSLSGSSVTVTPPDGTSALVECECGGTKISETINKTKQFSQCTKKEQCRAYRLQNDKWVYNSDKLPKAAGATLKGKLHVPFLLADGKCTVPLAPEPMITFGFRSVSLKLHPKNPTYLITRQLADEPHYTHELISEPAVRNFTVTEKGWEFVWGNHPPKRFWAQETAPGNPHGLPHEVITHYYHRYPMSTILGLSICAAIATVSVAASTWLFCRSRVACLTPYRLTPNARIPFCLAVLCCARTARA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| S: THR28 THR30 ASN31 TYR32 ILE33 TRP50 ASN52 ASN55 ASP102 TYR103 GLY104 GLY105 ASN106 ASP108 T: LEU27 PRO28 LYS29 GLN30 TYR31 TYR33 LYS49 ASP50 SER51 GLU52 SER66 ASN95 K: GLY183 SER184 SER185 ASP192 GLY193 GLY203 LYS206 SER208 THR210 ILE211 ASN212 LYS213 THR214 LYS215 GLN216 PHE217 SER218 GLN219 THR221 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
