Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 488 |
| PDBID: | 3FFD |
| Chains: | AB_P |
| Organism: | Mus musculus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.00 |
| Reference: | 10.1074/jbc.M900044200 |
| Antibody | |
| Antibody: | 3FFD Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Parathyroid hormone-related protein |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >3FFD_A|Chain A|Monoclonal antibody, heavy chain, Fab fragment|Mus musculus (10090) EVQLVESGGDLVKPGGSLKLSCAASGFTFSSYGMSWIRQTPDKRLEWVATISSGGSYTYYPDSVKGRFTISRDNAKNTLYLQMSSLKSEDTAMFYCARQTTMTYFAYWGQGTLVTVSAAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSPRPSETVTCNVAHPASSTKVDKKIEPA |
Light Chain: B
Mutation: NULL
| >3FFD_B|Chain B|Monoclonal antibody, light chain, Fab fragment|Mus musculus (10090) QLVLTQSSSASFSLGASAKLTCTLSSQHSTYTIEWYQQQPLKPPKYVMDLKQDGSHSTGDGIPDRFSGSSSGADRYLSISNIQPEDEAMYICGVGDTIKEQFVYVFGGGTKVTVLGEPKSTPTLTVFPPSSEELKENKATLVCLISNFSPSGVTVAWKANGTPITQGVDTSNPTKEGNKFMASSFLHLTSDQWRSHNSFTCQVTHEGDTVEKSLSPAECL |
Antigen
Chain: P
Mutation: NULL
| >3FFD_P|Chain C[auth P]|Parathyroid hormone-related protein|Homo sapiens (9606) AVSEHQLLHDKGKSIQDLRRRFFLHHLIAEIHTAEIRATSEVSPNSKPSPNTKNHPVRFGSDDEGRYLTQETNKVETYKEQPLKTPGKKKKGKPGKRKEQEKKKRRTR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: SER31 TYR32 GLY33 THR50 SER52 SER53 GLY54 SER56 TYR57 TYR59 GLN99 THR101 MET102 THR103 TYR104 B: THR30 TYR31 THR32 GLU34 ASP49 LYS51 GLN52 GLY95 ASP96 THR97 PHE102 P: SER14 GLN16 ASP17 ARG19 ARG20 ARG21 PHE23 LEU24 HIS26 LEU27 ILE28 GLU30 ILE31 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
