Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4873 |
| PDBID: | 7SEM |
| Chains: | BC_F |
| Organism: | Homo sapiens, human metapneumovirus |
| Method: | XRD |
| Resolution (Å): | 2.20 |
| Reference: | 10.1038/s41467-022-28931-3 |
| Antibody | |
| Antibody: | MPE8 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human metapneumovirus fusion glycoprotein F0 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >7SEM_B|Chain A[auth B]|MPE8 Fab heavy chain|Homo sapiens (9606) EVQLVESGGGLVKPGGSLRLSCAASGFTFSSYSMNWVRQAPGKGLEWVSSISASSSYSDYADSAKGRFTISRDNAKTSLFLQMNSLRAEDTAIYFCARARATGYSSITPYFDIWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCD |
Light Chain: C
Mutation: NULL
| >7SEM_C|Chain C|MPE8 Fab light chain|Homo sapiens (9606) QSVVTQPPSVSGAPGQRVTISCTGSSSNIGAGYDVHWYQQLPGTAPKLLIYDNNNRPSGVPDRFSASKSGTSASLAITGLQAEDEADYYCQSYDRSLSGVFGTGTKVTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHKSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: F
Mutation: NULL
| >7SEM_F|Chain B[auth F]|Fusion glycoprotein F0|Human metapneumovirus (162145) MSWKVVIIFSLLITPQHGLKESYLEESCSTITEGYLSVLRTGWYTNVFTLEVGDVENLTCADGPSLIKTELDLTKSALRELRTVSADQLAREEQIENPRRRRFVLGAIALGVATAAAVTAGVAIAKCIRLESEVTAIKNALKKTNEAVSTLGCGVRVLATAVRELKDFVSKNLTRAINKNKCDIPDLKMAVSFSQFNRRFLNVVRQFSDNAGITPAISLDLMTDAELARAVSNMPTSAGQIKLMLENRAMVRRKGFGILIGVYGSSVIYMVQLPIFGVIDTPCWIVKAAPSCSEKKGNYACLLREDQGWYCQNAGSTVYYPNEKDCETRGDHVFCDTAAGINVAEQSKECNINISTTNYPCKVSCGRNPISMVALSPLGALVACYKGVSCSIGSNRVGIIKQLNKGCSYITNQDADTVTIDNTVYQLSKVEGEQHVIKGRPVSSSFDPVKFPEDQFNVALDQCFESIENSQALVDQSNRILSSAEKGNTGGGGSGYIPEAPRDGQAYVRKDGEWVLLSTFLGRSLEVLFQGPGHHHHHHHHSAWSHPQFEK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: THR28 SER30 SER31 TYR32 ALA52 SER53 SER55 TYR56 ASN73 ARG96 ALA97 THR98 GLY99 C: GLY28 ALA29 GLY30 TYR31 TYR91 ARG93 F: LEU36 THR41 GLY42 TRP43 TYR44 ALA120 ARG156 SER232 ASN233 MET234 PRO235 THR236 SER237 ALA238 GLY239 GLN240 ILE241 LYS242 LEU243 ILE275 GLY277 VAL278 ILE279 ASP280 THR281 PRO282 GLN312 ALA314 GLY315 HIS332 GLU345 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
