Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4872 |
| PDBID: | 7SEG |
| Chains: | AB_D |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.16 |
| Reference: | 10.1038/s41375-021-01478-w |
| Antibody | |
| Antibody: | anti-CD16a Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Low affinity immunoglobulin gamma Fc region receptor III-A (CD16A) |
| Antigen mutation: | Yes |
| Durg Target: | P08637; O75015; |
Sequence information
Antibody
Heavy Chain: A
Mutation: NULL
| >7SEG_A|Chain B[auth A], D[auth H]|anti-CD16A Fab heavy chain|Homo sapiens (9606) MKKNIAFLLASMFVFSIATNAYAEVQLVQSGAEVKKPGESLKVSCKASGYTFTSYYMHWVRQAPGQGLEWMGAIEPMYGSTSYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARGSAYYYDFADYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCD |
Light Chain: B
Mutation: NULL
| >7SEG_B|Chain A[auth B], C[auth L]|anti-CD16a Fab light chain|Homo sapiens (9606) MKKNIAFLLASMFVFSIATNAYASYVLTQPSSVSVAPGQTATISCGGHNIGSKNVHWYQQRPGQSPVLVIYQDNKRPSGIPERFSGSNSGNTATLTISGTQAMDEADYYCQVWDNYSVLFGGGTKLTVLRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: D
Mutation: F161V
| >7SEG_D|Chain E[auth C], F[auth D]|Low affinity immunoglobulin gamma Fc region receptor III-A|Homo sapiens (9606) AGSRTEDLPKAVVFLEPQWYRVLEKDSVTLKCQGAYSPEDQSTQWFHNESLISSQASSYFIDAATVDDSGEYRCQTQLSTLSDPVQLEVHIGWLLLQAPRWVFKEEDPIHLRCHSWKNTALHKVTYLQNGKGRKYFHHNSDFYIPKATLKDSGSYFCRGLVGSKNVSSETVQITITQGGN |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: GLY29 SER30 LYS31 ASN32 GLN50 ASP51 TRP91 ASN93 TYR94 A: THR28 THR30 SER31 TYR32 TYR33 MET53 TYR54 GLY55 SER56 THR57 ALA97 TYR98 TYR99 D: PHE8 LEU9 GLU10 PRO11 GLN12 SER21 LYS25 GLN91 GLU100 PRO102 HIS104 ARG106 HIS108 ASN112 LYS128 PHE130 HIS132 ASN133 SER134 ASP135 PHE136 TYR137 PRO139 LYS140 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
