Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4869 |
| PDBID: | 7SD3 |
| Chains: | HG_F |
| Organism: | Homo sapiens, HIV whole-genome vector AA1305#18 |
| Method: | EM |
| Resolution (Å): | 3.67 |
| Reference: | 10.1038/s41467-022-34008-y |
| Antibody | |
| Antibody: | 4E10 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | HIV-1 envelope glycoprotein - gp41 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7SD3_H|Chain A[auth H], C[auth M], E[auth N]|4E10 Fab light chain|Homo sapiens (9606) EIVLTQSPGTQSLSPGERATLSCRASQSVGNNKLAWYQQRPGQAPRLLIYGASSRPSGVADRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYGQSLSTFGQGTKVEVKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE |
Light Chain: G
Mutation: NULL
| >7SD3_G|Chain B[auth G], D[auth I], F[auth K]|4E10 Fab heavy chain|Homo sapiens (9606) QVQLVQSGAEVKRPGSSVTVSCKASGGSFSTYALSWVRQAPGRGLEWMGGVIPLLTITNYAPRFQGRITITADRSTSTAYLELNSLRPEDTAVYYCAREGTTGWGWLGKPIGAFAHWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSC |
Antigen
Chain: F
Mutation: NULL
| >7SD3_F|Chain H[auth B], J[auth D], L[auth F]|Transmembrane protein gp41|HIV whole-genome vector AA1305#18 (672471) AVGIGAVFLGFLGAAGSTMGAASMTLTVQARNLLSGIVQQQSNLLRAPEAQQHLLKLTVWGIKQLQARVLAVERYLRDQQLLGIWGCSGKLICCTNVPWNSSWSNRNLSEIWDNMTWLQWDKEISNYTQIIYGLLEESQNQQEKNEQDLLALDKWASLWNWFDISNWLWYIKL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: ASN30 LYS32 TYR91 GLY92 GLN93 SER94 G: THR31 TYR32 ALA33 LEU53 LEU54 ILE56 ASN58 GLU95 F: LEU661 TRP670 TRP672 PHE673 ASP674 ILE675 SER676 ASN677 LEU679 TRP680 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
