Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4862 |
| PDBID: | 7SCO |
| Chains: | HL_B |
| Organism: | Influenza A virus (A/South Canterbury/35/2000(H1N1)), Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.37 |
| Reference: | 10.1016/j.immuni.2022.07.006 |
| Antibody | |
| Antibody: | 310-39G10 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Influenza A virus South Canterbury/35/2000 H1N1 NC99 hemagglutinin |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7SCO_H|Chain C[auth E], G[auth H], K[auth J]|310-39G10 Fab, Heavy Chain|Homo sapiens (9606) QVQLVQSGAEVKKPGSSVKVSCKASGGTFSRYTISWVRQAPGQGLEWMGRISPIAGLENYAQKFQGRVTITADISTSTAYMELSSLRSDDTAVYYCAGSSGYYQPPPYWGQGTLVTVSS |
Light Chain: L
Mutation: NULL
| >7SCO_L|Chain D[auth F], H[auth K], L|310-39G10 Fab, Light Chain|Homo sapiens (9606) EIVLTQAPFSLSLSPGERATLSCRASQSVSSSSLAWYHQKPGQAPRLLIYGASSRATGIPDRFSGSGSGTDFTLTISRLEPEDFSLYYCQQYGSSPLTFGQGTRLEIK |
Antigen
Chain: B
Mutation: NULL
| >7SCO_B|Chain B, F[auth D], J[auth I]|Hemagglutinin HA2 chain|Influenza A virus (strain A/New Zealand:South Canterbury/35/2000 H1N1) (363066) RETRGLFGAIAGFIEGGWTGMVDGWYGYHHQNEQGSGYAADQKSTQNAINGITNKVNSVIEKMNTQFTAVGKEFNKLERRMENLNKKVDDGFLDIWTYNAELLVLLENERTLDFHDSNVKNLYEKVKSQLKNNAKEIGNGCFEFYHKCNNECMESVKNGTYDYPKYSEESKLNREKIDGVKYIPEAPRDGQAYVRKDGEWVLLSTFLGSGLNDIFEAQKIEWHEGHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: THR535 SER537 ARG538 ARG557 SER559 ILE561 ALA562 LEU564 GLU565 ASN566 ILE581 SER607 GLY608 TYR609 TYR610 L: SER651 SER652 SER654 GLY714 SER716 B: MET347 VAL348 ASP349 GLY350 TRP351 TYR364 ALA366 GLN368 THR371 GLN372 ILE375 ILE378 THR379 ASN380 VAL382 ASN383 ILE386 GLU387 GLU480 ASN484 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
