Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4788 |
| PDBID: | 7RTH |
| Chains: | PO_o |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.19 |
| Reference: | 10.1073/pnas.2115435118 |
| Antibody | |
| Antibody: | NabFab Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Lyso-Nb_4 nanobody |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: P
Mutation: NULL
| >7RTH_P|Chain B, D, F, H, J, L, N, P, R|Fragment Antigen-Binding Heavy Chain|Homo sapiens (9606) EISEVQLVESGGGLVQPGGSLRLSCAASGFNFSYYSIHWVRQAPGKGLEWVAYISSSSSYTSYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARGYQYWQYHASWYWNGGLDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHT |
Light Chain: O
Mutation: NULL
| >7RTH_O|Chain A, C, E, G, I, K, M, O, Q|Fragment Antigen-Binding Light Chain|Homo sapiens (9606) SDIQMTQSPSSLSASVGDRVTITCRASQSVSSAVAWYQQKPGKAPKLLIYSASSLYSGVPSRFSGSRSGTDFTLTISSLQPEDFATYYCQQSSSSLITFGQGTKVEIKRTVAAPSVFIFPPSDSQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: o
Mutation: NULL
| >7RTH_o|Chain AA[auth a], S[auth c], T[auth e], U[auth g], V[auth i], W[auth k], X[auth m], Y[auth o], Z[auth q]|Nanobody|Homo sapiens (9606) GSQRQLVESGGGLVQPGGSLRLSCAASGSTDSIEYMTWFRQAPGKEREGVAALYTHTGNTYYADSVKGRFIISRDKAKNMVYLQMNSLEPEDTAVYYCGATRKYVPVRFALDQSSYDYWGKGTPVTVSS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| O: LEU46 TYR49 SER50 SER52 SER53 TYR55 SER56 ARG66 P: SER0 VAL2 ASN28 TYR31 TYR32 TYR56 ARG94 TYR96 GLN97 TYR98 ASP101 TYR102 o: VAL5 GLU6 GLY8 GLY9 GLY10 LEU11 GLN39 ALA40 PRO41 GLY42 LYS43 GLU44 ARG45 THR87 ALA88 VAL89 TYR91 TYR100 ASP101 TRP103 GLY104 LYS105 GLY106 PRO108 VAL109 THR110 VAL111 SER112 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
