Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4769 |
| PDBID: | 7RNN |
| Chains: | C_D |
| Organism: | Vicugna pacos, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 2.86 |
| Reference: | 10.7554/eLife.67115 |
| Antibody | |
| Antibody: | Nb.C1 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Acid-sensing ion channel 1 |
| Antigen mutation: | No |
| Durg Target: | P78348 |
Sequence information
Antibody
Chain: C
Mutation: NULL
| >7RNN_C|Chain A[auth C]|Nanobodies Nb.C1|Vicugna pacos (30538) QVQLVESGGGLVQPRGSLRLSCAASGFTFSRAAMSWYRQAPGKEREMVSTIGSFGVSTNYSDSVKGRFTISRDNAKNTVYLHMNSLKPEDTAVYYCNARYRSSYPWGQGTQVTVSS |
Antigen
Chain: D
Mutation: NULL
| >7RNN_D|Chain B[auth D]|Acid-sensing ion channel 1|Homo sapiens (9606) MELKAEEEEVGGVQPVSIQAFASSSTLHGLAHIFSYERLSLKRALWALCFLGSLAVLLCVCTERVQYYFHYHHVTKLDEVAASQLTFPAVTFCNLNEFRFSQVSKNDLYHAGELLALLNNRYEIPDTQMADEKQLEILQDKANFRSFKPKPFNMREFYDRAGHDIRDMLLSCHFRGEVCSAEDFKVVFTRYGKCYTFNSGRDGRPRLKTMKGGTGNGLEIMLDIQQDEYLPVWGETDETSFEAGIKVQIHSQDEPPFIDQLGFGVAPGFQTFVACQEQRLIYLPPPWGTCKAVTMDSDLDFFDSYSITACRIDCETRYLVENCNCRMVHMPGDAPYCTPEQYKECADPALDFLVEKDQEYCVCEMPCNLTRYGKELSMVKIPSKASAKYLAKKYNKSEQYIGENILVLDIFFEVLNYETIEQKKAYEIAGLLGDIGGQMGLFIGASILTVLELFDYAYEVIKHKLCRRGKCQKEAKRSSADKGVALSLDDVKRHNPCEGLRGHPAGMTYAANILPHHPARGTFEDFTC |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: PHE31 THR32 ARG35 ALA36 ALA37 GLY56 SER57 PHE58 VAL60 SER61 TYR104 ARG105 SER107 TYR108 D: LYS291 ASP296 SER297 ASP298 LEU299 ASP300 PHE301 TYR305 CYS310 ASP313 CYS314 ARG317 TYR318 VAL320 GLU321 ASN322 ASN324 ASP333 TYR360 CYS361 VAL362 GLU364 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
