Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4760 |
| PDBID: | 7RK1 |
| Chains: | C_A |
| Organism: | Human astrovirus 8, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.05 |
| Reference: | 10.1128/JVI.01415-21 |
| Antibody | |
| Antibody: | 3E8 scFv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | human astrovirus serotype 8 capsid spike VP70 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: C
Mutation: NULL
| >7RK1_C|Chain B[auth C], D|scFv 3E8|Mus musculus (10090) DVQLQESGPGLVKPSQSLSLTCSVTGYSITSGYYWNWIRQFPGNKLEWMGYISYDGSNNYNPSLKNRISITRDTSKNQFFLKLNSVTTEDTATYYCATFYDGYDYWGQGTTLTVSSGGSGGGGSGGGGSGGGGSDIVMTQSHKFMSTSVGDRVSITCKASQDVSTAVAWYQQKPGQSPKLLIYWASTRHTGVPDRFTGSGSGTDYTLTISSVQAEDLALYYCQQHYSTPFTFGSGTKLEIKRASLVPR |
Antigen
Chain: A
Mutation: NULL
| >7RK1_A|Chain A, C[auth B]|Capsid polyprotein VP70|Human astrovirus-8 (43358) MGGEQFRVLLTVGPPMAPNTANSQNWVNKTIVPPENQYTVKIGIDLEHYTTMQGFTPVESVSWYTADFQPSDEPSPIPGLYARVNNTKKADVYGVQQFKSSHTNNRHQITSVFLVRVTTSFQVINYTSYFIRGAESGSNVSNLKIRDQTYHTPLQFTQGKWYLLTSTVMHDGPTSSGWVWMNQELTNNIAYRVDPGMMYLITPPPAASQLYFELHTVLPQAAAELALVPR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: TYR27 SER31 GLY32 TYR33 TYR34 ASN36 TRP48 TYR51 TYR54 ASN59 PHE99 TYR100 ASP101 SER164 THR165 TRP184 THR187 HIS225 TYR226 SER227 THR228 PHE230 A: PRO459 GLU461 ASN462 GLN463 TYR464 LYS467 VAL510 ASN511 ASN512 THR513 LYS515 PHE547 GLN548 VAL549 ILE550 TYR576 HIS577 PRO579 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
