Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4755 |
| PDBID: | 7REW |
| Chains: | HL_I |
| Organism: | Homo sapiens, Macaca fascicularis |
| Method: | XRD |
| Resolution (Å): | 2.10 |
| Reference: | 10.1016/j.jbc.2021.101533 |
| Antibody | |
| Antibody: | MMAb3 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Macaca fascicularis nonhistone protein (NHP) IL-13 |
| Antigen mutation: | No |
| Durg Target: | P35225 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7REW_H|Chain A, C[auth H]|anti-cyno interleukin 13 Fab heavy chain|Homo sapiens (9606) EVQLLESGGGLVQPGGSLRLSCAASGFTFSSYAMSWVRQAPGKGLEWVSAFSGWDVSTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCVQDGLGPYFYNYGMDVWGQGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCD |
Light Chain: L
Mutation: NULL
| >7REW_L|Chain B, D[auth L]|anti-cyno interleukin 13 Fab light chain|Homo sapiens (9606) SYELTQPPSVSVSPGQTASITCSGDKLGDKYSFWFQQKPGQSPVLVIYHDSKRPSGIPERFSGSNSGDTATLTISGTRAMDEADYYCQAWDSSTYVFGTGTKVTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: I
Mutation: NULL
| >7REW_I|Chain E[auth G], F[auth I]|IL13|Macaca fascicularis (9541) GPVPPSTALKELIEELVNITQNQKAPLCNGSMVWSINLTAGVYCAALESLINVSGCSAIEKTQRMLNGFCPHKVSAGQFSSLRVRDTKIEVAQFVKDLLVHLKKLFREGQFN |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: THR28 SER30 SER31 TYR32 GLY53 TRP54 LEU101 GLY102 PRO103 TYR104 TYR106 ASN107 TYR108 MET110 ASP111 L: GLY28 ASP29 LYS30 TYR31 LEU45 TYR48 HIS49 ASP50 LYS52 ARG53 PRO54 SER55 ASN65 I: THR8 ALA9 LEU10 GLU12 GLU61 LYS62 GLN64 ARG65 MET66 ASN68 GLY69 PHE70 CYS71 PRO72 HIS73 LYS74 VAL75 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
