Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4748 |
| PDBID: | 7RD5 |
| Chains: | DC_E |
| Organism: | Mus musculus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.60 |
| Reference: | 10.1016/j.str.2021.10.007 |
| Antibody | |
| Antibody: | 1C12 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Tspan15 large extracellular loop (Tspan15 LEL) |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: D
Mutation: NULL
| >7RD5_D|Chain B, E[auth D]|1C12 Fab Heavy Chain|Mus musculus (10090) QVHLQQSGPELVKPGASVKISCKASGYTFSDYYINWVRQRPGQGLEWIGWIFPGSGNTYYNKEFKVKATLTVDKSSSTAHMFLSSLTSEDSAVYFCVRRYDYDGFAFWGLGTLVTVSAAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSQTVTCNVAHPASSTKVDKKIVPRDCLEVLFQ |
Light Chain: C
Mutation: NULL
| >7RD5_C|Chain A, D[auth C]|1C12 Fab Light Chain|Mus musculus (10090) DIQMQQSPSSLSASLGDTITITCHASQNINAWLSWYQQKPGNIPKLLIYKASNLYTGVPSRFSGSGSGTRFTLTISSLQPEDIATYYCQQGQSSPYTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNECLEVLFQ |
Antigen
Chain: E
Mutation: NULL
| >7RD5_E|Chain C[auth E], F|Tetraspanin-15|Homo sapiens (9606) TFRQQTIDFLNDNIRRGIENYYDDLDFKNIMDFVQKKFKCCGGEDYRDWSKNQYHDCSAPGPLACGVPYTCCIRDTTEVVNTMCGYKTIDKERFSVQDVIYVRGCTNAVIIWFMDNLEVLFQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: TRP32 LYS50 GLY91 GLN92 SER93 SER94 TYR96 D: ASP31 TYR32 TYR33 TRP50 PHE52 SER55 ASN57 THR58 TYR59 ARG99 TYR100 ASP101 TYR102 ASP103 E: ASP159 ARG161 SER164 ASP170 CYS171 SER172 CYS179 LYS201 THR202 ILE203 ASP204 LYS205 GLU206 ARG207 PHE208 SER209 VAL210 VAL213 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
