Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4744 |
| PDBID: | 7RBU |
| Chains: | HL_B |
| Organism: | Severe acute respiratory syndrome coronavirus 2, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.70 |
| Reference: | 10.1128/mbio.03580-21 |
| Antibody | |
| Antibody: | PVI.V6-14 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | SARS-CoV-2 spike glycoprotein NTD |
| Antigen mutation: | No |
| Durg Target: | P0DTC2 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7RBU_H|Chain B[auth H]|PVI.V6-14 Fab heavy chain, variable region|Homo sapiens (9606) QVQLQESGPGLVKPSETLSLTCTVSGGSISSSSYYWGWIRQPPGKGLEWIGSIYYSGSTYYNPSLKSRVTISVDTSKNQFSLKLSSVTAADTAVYYCARCRPEYYFGSGSYLDFDYWGQGTLVTVSS |
Light Chain: L
Mutation: NULL
| >7RBU_L|Chain C[auth L]|PVI.V6-14 Fab light chain, variable region|Homo sapiens (9606) DIQMTQSPSSVSASVGDRVTITCRASQGISSWLAWYQQKPGKAPKLLIYAASSLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQANSFPLTFGGGTKVEIK |
Antigen
Chain: B
Mutation: NULL
| >7RBU_B|Chain A[auth B]|Spike protein S1|Severe acute respiratory syndrome coronavirus 2 (2697049) QCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFT |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition H: SER31 SER33 TYR35 TYR54 SER56 TYR60 ARG101 GLU103 TYR104 TYR105 PHE106 GLY107 SER108 GLY109 SER110 TYR111 LEU112 ASP113 L: TRP32 ALA50 ALA91 ASN92 PHE94 B: ASN99 ILE101 ARG102 TRP104 ILE119 ASN121 VAL126 ILE128 TYR170 VAL171 SER172 GLN173 PRO174 PHE175 LEU176 MET177 ASP178 LYS182 LYS187 ASN188 ARG190 PHE192 HIS207 PRO209 PRO225 LEU226 VAL227 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
