Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4741 |
| PDBID: | 7RAH |
| Chains: | BA_E |
| Organism: | Mus musculus, Bordetella pertussis |
| Method: | XRD |
| Resolution (Å): | 2.60 |
| Reference: | 10.1371/journal.ppat.1009920 |
| Antibody | |
| Antibody: | M1H5 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Bifunctional adenylate cyclase toxin/hemolysin CyaA |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >7RAH_B|Chain B|M1H5 Fab Heavy Chain|Mus musculus (10090) EVNLVESGGDLVKPGGSLKLSCAASGFTFSSYGMSWVRQTPDKRLEWVATISSGGTYTYYPDSVKGRFTISRDNAKNTLYLQMSSLKSEDTAMYYCAREIMRGGGYYFDYWSQGTTLTVSSRSTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKGLEVLFQ |
Light Chain: A
Mutation: NULL
| >7RAH_A|Chain A|M1H5 Fab Light Chain|Mus musculus (10090) DIQMIQSTSSLSASLGDRVTISCRASQDISNYLNWYQQKPDGTVKLLIYYTSRLHSGVPSRFSGSGSGTDYSLTISNLEQEDIATYFCQQGNTLPYTFGGGTKLEIKRTADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC |
Antigen
Chain: E
Mutation: NULL
| >7RAH_E|Chain E|Bifunctional adenylate cyclase toxin/hemolysin CyaA,Bifunctional adenylate cyclase toxin/hemolysin CyaA|Bordetella pertussis (520) GPGSGGAGNDSITGNAHDNFLAGGSGDDRLDGGAGNDTLVGGEGQNTVIGGAGDDVFLQDLGVWSNQLDGGAGVDTVKYNVHQPSEERLERMGDTGIHADLQKGTVEKWPALNLFSVDHVKNIENLHGSRLNDRIAGDDQDNELWGHDGNDTIRGRGGDDILRGGLGLDTLYGEDGNDIFLQDDETVSDDIDGGAGLDTVDYSAMIHPGRIVAPHEYGFGIEADLSREWVRKASALGVDYYDNVRNVENVIGTSMKDVLIGDAQANTLMGQGGDDTVRGGDGDDLLFGGDGNDMLYGDAGNDTLYGGLGDDTLEGGAGNDWFGQTQAREHDVLRGGDGVDTYLFGVGYGHDTIYESGGGHDTIRINAGADQLWFARQGNDLEIRILGTDDALTVHDWYRDADHRVEIIHAANQAVDQAGIEKLVEAMAQYPDPGAAAAAPPAARVPDTLMQSLAVNWR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: SER30 TYR32 TYR49 ARG53 SER56 ASN92 THR93 TYR96 B: SER30 SER31 TYR32 GLY33 THR50 SER52 GLY53 THR55 TYR56 TYR58 GLU95 ILE96 MET97 ARG98 GLY99 E: SER1198 TYR1227 PHE1229 GLU1232 ASP1234 LEU1235 SER1236 ARG1237 GLU1238 TRP1239 ARG1241 SER1244 GLY1247 ASP1249 TYR1251 ASN1253 ARG1255 ASN1256 VAL1268 ILE1270 ASP1272 ALA1273 GLN1274 THR1286 ARG1288 TYR1306 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
