Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4725 |
| PDBID: | 7R8A |
| Chains: | CD_B |
| Organism: | Homo sapiens, Mus musculus |
| Method: | EM |
| Resolution (Å): | 2.90 |
| Reference: | 10.1073/pnas.2110483118 |
| Antibody | |
| Antibody: | 2C7 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | ATP-binding cassette sub-family G member 8 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: C
Mutation: NULL
| >7R8A_C|Chain C|2C7 Fab heavy chain|Mus musculus (10090) MGWSCIILFLVATATGVHSEVKLVESGGGLVQPGGSLRLSCATSGFTFSEFFMEWVRQPPGKRLEWVAVSRNEANDYTTDYSASVKGRFIVSRDTSQNILYLQMNALRAEDTAIYYCARDAWMGFDYWGQGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSCDKTH |
Light Chain: D
Mutation: NULL
| >7R8A_D|Chain D|2C7 Fab light chain|Mus musculus (10090) MGWSCIILFLVATARTGVHSDIQMTQSPSSLSASLGERVSLTCRASQEISGYLSWLQQKPDGTIQRLIYAAFSLDSGVPKRFSGSRSGSDYSLTISSLESEDLAHYYCLQYASYPCTFGGGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: B
Mutation: NULL
| >7R8A_B|Chain B|ATP-binding cassette sub-family G member 8|Homo sapiens (9606) MAGKAAEERGLPKGATPQDTSGLQDRLFSSESDNSLYFTYSGQPNTLEVRDLNYQVDLASQVPWFEQLAQFKMPWTSPSCQNSCELGIQNLSFKVRSGQMLAIIGSSGCGRASLLDVITGRGHGGKIKSGQIWINGQPSSPQLVRKCVAHVRQHNQLLPNLTVRETLAFIAQMRLPRTFSQAQRDKRVEDVIAELRLRQCADTRVGNMYVRGLSGGERRRVSIGVQLLWNPGILILDEPTSGLDSFTAHNLVKTLSRLAKGNRLVLISLHQPRSDIFRLFDLVLLMTSGTPIYLGAAQHMVQYFTAIGYPCPRYSNPADFYVDLTSIDRRSREQELATREKAQSLAALFLEKVRDLDDFLWKAETKDLDEDTCVESSVTPLDTNCLPSPTKMPGAVQQFTTLIRRQISNDFRDLPTLLIHGAEACLMSMTIGFLYFGHGSIQLSFMDTAALLFMIGALIPFNVILDVISKCYSERAMLYYELEDGLYTTGPYFFAKILGELPEHCAYIIIYGMPTYWLANLRPGLQPFLLHFLLVWLVVFCCRIMALAAAALLPTFHMASFFSNALYNSFYLAGGFMINLSSLWTVPAWISKVSFLRWCFEGLMKIQFSRRTYKMPLGNLTIAVSGDKILSVMELDSYPLYAIYLIVIGLSGGFMVLYYVSLRFIKQKPSQDWASNSLEVLFQGPNVDSKRRWKKNFIAVSAANRFKKISSSGAL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition C: PHE52 ARG71 ASN72 ALA74 ASN75 ASP76 THR78 ASP80 TRP122 MET123 D: ILE22 GLN47 GLU48 ILE49 SER50 TYR52 ARG86 TYR111 ALA112 SER113 TYR114 B: GLU31 SER32 ASP33 ASN34 SER35 LEU36 TYR37 THR39 ARG164 LYS186 GLU189 ASP190 ILE192 ALA193 ARG198 GLN199 ALA201 ASP202 THR203 LYS260 GLY261 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
