Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4710 |
| PDBID: | 7R1Z |
| Chains: | E_A |
| Organism: | Vicugna pacos, Rattus norvegicus |
| Method: | XRD |
| Resolution (Å): | 1.94 |
| Reference: | 10.1371/journal.pone.0269281 |
| Antibody | |
| Antibody: | H11 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | collapsed human Activity-regulated cytoskeleton-associated protein (hArc) CTD |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: E
Mutation: NULL
| >7R1Z_E|Chain A[auth E]|NbArc-H11|Vicugna pacos (30538) GSEVQLLESGGGLVQAGDSLRLSCAASGRTFSAYAMGWFRQAPGKEREFVAAISWSGNSTYYADSVKGRFTISRDNAKNTVYLQMNSLKPEDTAIYYCAARKPMYRVDISKGQNYDYWGQGTQVTVSS |
Antigen
Chain: A
Mutation: NULL
| >7R1Z_A|Chain B[auth A]|Activity-regulated cytoskeleton-associated protein|Rattus norvegicus (10116) MHHHHHHLESTSLYKKAGSENLYFQSPGVDTQIFEDPREFLSHLEEYLRQVGGSEEYWLSQIQNHMNGPAKKWWEFKQGSVKNWVEFKKEFLQYSEGTLSREAIQRELDLPQKQGEPLDQFLWRKRDLYQTLYVDADEEEIIQYVVGTLQPKLKRFLRHPLPKTLEQLIQRGMEVQDDLEQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| E: ARG29 THR30 SER32 ALA33 TYR34 ALA35 PHE49 SER54 TRP55 SER56 ASN58 SER59 TYR61 TYR62 ASN76 ARG101 LYS102 PRO103 MET104 TYR105 ARG106 VAL107 ASP108 ILE109 SER110 A: GLN212 ILE213 PHE214 GLU215 ASP216 PRO217 GLU219 PHE220 SER222 HIS223 LEU224 GLU226 TYR227 GLN230 VAL231 ASN244 HIS245 MET246 ASN247 PRO249 ALA250 PHE271 TYR274 SER275 LEU279 LYS293 GLN294 GLY295 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
