Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 470 |
| PDBID: | 3EOA |
| Chains: | BA_J |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.80 |
| Reference: | 10.1073/pnas.0810844106 |
| Antibody | |
| Antibody: | Efalizumab Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | Efalizumab(Approved) |
| Antigen | |
| Antigen: | Integrin alpha-L |
| Antigen mutation: | No |
| Durg Target: | P20701 |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >3EOA_B|Chain C[auth B], D[auth H]|Efalizumab Fab fragment, heavy chain|Homo sapiens (9606) EVQLVESGGGLVQPGGSLRLSCAASGYSFTGHWMNWVRQAPGKGLEWVGMIHPSDSETRYNQKFKDRFTISVDKSKNTLYLQMNSLRAEDTAVYYCARGIYFYGTTYFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVE |
Light Chain: A
Mutation: NULL
| >3EOA_A|Chain A, B[auth L]|Efalizumab Fab fragment, light chain|Homo sapiens (9606) DIQMTQSPSSLSASVGDRVTITCRASKTISKYLAWYQQKPGKAPKLLIYSGSTLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQQHNEYPLTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: J
Mutation: NULL
| >3EOA_J|Chain E[auth I], F[auth J]|Integrin alpha-L|Homo sapiens (9606) GNVDLVFLFDGSMSLQPDEFQKILDFMKDVMKKLSNTSYQFAAVQFSTSYKTEFDFSDYVKRKDPDALLKHVKHMLLLTNTFGAINYVATEVFREELGARPDATKVLIIITDGEATDSGNIDAAKDIIRYIIGIGKHFQTKESQETLHKFASKPASEFVKILDTFEKLKDLFTELQKKIYV |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: LYS31 TYR49 SER50 THR53 SER56 B: SER28 THR30 GLY31 HIS32 TRP33 HIS52 SER54 ASP55 GLU57 LYS74 ILE100 TYR101 PHE102 TYR103 GLY104 THR105 TYR107 J: PRO144 ASP145 PHE147 GLN148 LEU151 ASP152 LYS155 ASP191 ASP193 ALA194 LYS197 HIS198 VAL199 LYS200 HIS201 LEU203 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
