Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4687 |
| PDBID: | 7QBF |
| Chains: | B_A |
| Organism: | Homo sapiens, Vicugna pacos |
| Method: | XRD |
| Resolution (Å): | 1.85 |
| Reference: | 10.1096/fj.202101376RR |
| Antibody | |
| Antibody: | TC-Nb34 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Transcobalamin-2 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >7QBF_B|Chain C[auth B]|Anti-transcobalamin-2 nanobody TC-Nb34|Vicugna pacos (30538) QWQLVESGGGLVQPGGSLRLSCAASGSTFSSYAMGWYRQAPGKECELVAAISRAGGSTNYADSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCNAAAEGETGSNWSLCEEYDYWGKGTRVTVSSHHHHHHEPEA |
Antigen
Chain: A
Mutation: NULL
| >7QBF_A|Chain A|Transcobalamin-2|Homo sapiens (9606) EMCEIPEMDSHLVEKLGQHLLPWMDRLSLEHLNPSIYVGLRLSSLQAGTKEDLYLHSLKLGYQQCLLGSAFSEDDGDCQGKPSMGQLALYLLALRANCEFVRGHKGDRLVSQLKWFLEDEKRAIGHDHKGHPHTSYYQYGLGILALCLHQKRVHDSVVDKLLYAVEPFHQGHHSVDTAAMAGLAFTCLKRSNFNPGRRQRITMAIRTVREEILKAQTPEGHFGNVYSTPLALQFLMTSPMRGAELGTACLKARVALLASLQDGAFQNALMISQLLPVLNHKTYIDLIFPDCLAPRVMLEPAAETIPQTQEIISVTLQVLSLLPPYRQSISVLAGSTVEDVLKKAHELGGFTYETQASLSGPYLTSVMGKAAGEREFWQLLRDPNTPLLQGIADYRPKDGETIELRLVSW |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: LEU4 VAL5 CYS22 ALA24 SER25 GLY26 SER27 THR28 PHE29 SER30 SER31 TYR32 MET34 ARG53 CYS96 ASN97 ALA98 ALA99 ALA100 TRP109 SER110 LEU111 GLU113 GLU114 TYR115 ASP116 TYR117 LYS120 A: HIS126 ASP127 HIS128 LYS129 ASP159 TYR163 VAL165 GLU166 PRO167 PHE168 HIS169 GLN170 GLY171 HIS172 PRO195 GLY196 ARG197 ARG198 GLN199 ARG200 MET203 ALA204 ARG206 THR207 GLU210 GLU211 LYS214 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
