Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4686 |
| PDBID: | 7QBE |
| Chains: | F_C |
| Organism: | Homo sapiens, Vicugna pacos |
| Method: | XRD |
| Resolution (Å): | 3.00 |
| Reference: | 10.1096/fj.202101376RR |
| Antibody | |
| Antibody: | TC-Nb11 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Transcobalamin-2 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: F
Mutation: NULL
| >7QBE_F|Chain E, F|Anti-transcobalamin-2 nanobody TC-Nb11|Vicugna pacos (30538) QLQLVESGGGLVQAGGSLRLSCTASGRTGTMGWFRQGPGKEREFVASHKWVAGSTYYADSVKGRFTISRDNAKNTLYLQMNSLKSEDTAVYYCAASSQIFYGATTSIKDFNSWGKGTRVTVSSHHHHHHEPEA |
Antigen
Chain: C
Mutation: NULL
| >7QBE_C|Chain A, C|Transcobalamin-2|Homo sapiens (9606) EMCEIPEMDSHLVEKLGQHLLPWMDRLSLEHLNPSIYVGLRLSSLQAGTKEDLYLHSLKLGYQQCLLGSAFSEDDGDCQGKPSMGQLALYLLALRANCEFVRGHKGDRLVSQLKWFLEDEKRAIGHDHKGHPHTSYYQYGLGILALCLHQKRVHDSVVDKLLYAVEPFHQGHHSVDTAAMAGLAFTCLKRSNFNPGRRQRITMAIRTVQEEILKAQTPEGHFGNVYSTPLALQFLMTSPMRGAELGTACLKARVALLASLQDGAFQNALMISQLLPVLNHKTYIDLIFPDCLAPRVMLEPAAETIPQTQEIISVTLQVLSLLPPYRQSISVLAGSTVEDVLKKAHELGGFTYETQASLSGPYLTSVMGKAAGEREFWQLLRDPNTPLLQGIADYRPKDGETIELRLVSW |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| F: LEU24 ARG49 THR50 GLY51 PHE66 LYS71 TRP72 VAL73 ALA74 GLY75 SER76 TYR78 TYR79 ALA80 SER119 GLN120 ILE121 PHE122 TYR123 GLY124 ALA125 THR126 THR127 SER128 LYS130 ASP131 ASN133 C: ALA164 VAL165 GLU166 PRO167 PHE168 GLN170 HIS172 HIS173 SER174 VAL175 ALA178 VAL208 GLU211 ILE212 LEU213 LYS214 ALA215 GLN216 THR217 PRO218 GLY223 ASN224 LYS251 PRO383 ASN384 THR385 PRO386 LEU388 SER408 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
