Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4685 |
| PDBID: | 7QBE |
| Chains: | E_A |
| Organism: | Homo sapiens, Vicugna pacos |
| Method: | XRD |
| Resolution (Å): | 3.00 |
| Reference: | 10.1096/fj.202101376RR |
| Antibody | |
| Antibody: | TC-Nb11 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Transcobalamin-2 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: E
Mutation: NULL
| >7QBE_E|Chain E, F|Anti-transcobalamin-2 nanobody TC-Nb11|Vicugna pacos (30538) QLQLVESGGGLVQAGGSLRLSCTASGRTGTMGWFRQGPGKEREFVASHKWVAGSTYYADSVKGRFTISRDNAKNTLYLQMNSLKSEDTAVYYCAASSQIFYGATTSIKDFNSWGKGTRVTVSSHHHHHHEPEA |
Antigen
Chain: A
Mutation: NULL
| >7QBE_A|Chain A, C|Transcobalamin-2|Homo sapiens (9606) EMCEIPEMDSHLVEKLGQHLLPWMDRLSLEHLNPSIYVGLRLSSLQAGTKEDLYLHSLKLGYQQCLLGSAFSEDDGDCQGKPSMGQLALYLLALRANCEFVRGHKGDRLVSQLKWFLEDEKRAIGHDHKGHPHTSYYQYGLGILALCLHQKRVHDSVVDKLLYAVEPFHQGHHSVDTAAMAGLAFTCLKRSNFNPGRRQRITMAIRTVQEEILKAQTPEGHFGNVYSTPLALQFLMTSPMRGAELGTACLKARVALLASLQDGAFQNALMISQLLPVLNHKTYIDLIFPDCLAPRVMLEPAAETIPQTQEIISVTLQVLSLLPPYRQSISVLAGSTVEDVLKKAHELGGFTYETQASLSGPYLTSVMGKAAGEREFWQLLRDPNTPLLQGIADYRPKDGETIELRLVSW |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| E: ARG49 PHE66 LYS71 TRP72 VAL73 GLY75 SER76 TYR78 TYR79 ALA80 LYS84 SER119 GLN120 ILE121 PHE122 TYR123 GLY124 ALA125 THR126 THR127 SER128 ASP131 ASN133 A: ALA164 VAL165 GLU166 PRO167 PHE168 GLN170 HIS172 HIS173 SER174 VAL175 ALA178 GLU211 LEU213 LYS214 ALA215 GLN216 THR217 PRO218 GLU219 GLY223 LYS251 ASN384 THR385 PRO386 LEU388 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
