Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4684 |
| PDBID: | 7QBD |
| Chains: | E_B |
| Organism: | Homo sapiens, Vicugna pacos |
| Method: | XRD |
| Resolution (Å): | 4.18 |
| Reference: | 10.1096/fj.202101376RR |
| Antibody | |
| Antibody: | TC-Nb26 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Transcobalamin-2 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: E
Mutation: NULL
| >7QBD_E|Chain E|Antitranscobalamin-nanobody TC-Nb26|Vicugna pacos (30538) QVQLVESGGGLVQAGESLRLSCAASGRTFAMAWFRQAPGKEREFVAVRGWLGVTTYYADSVKGRFTISRDNAKNTLDLQMNSLKPEDTAVYYCAAGQYSSSLYDRETEYNYWGQGTRVTVSSHHHHHHEPEA |
Antigen
Chain: B
Mutation: NULL
| >7QBD_B|Chain A, B|Transcobalamin-2|Homo sapiens (9606) EMCEIPEMDSHLVEKLGQHLLPWMDRLSLEHLNPSIYVGLRLSSLQAGTKEDLYLHSLKLGYQQCLLGSAFSEDDGDCQGKPSMGQLALYLLALRANCEFVRGHKGDRLVSQLKWFLEDEKRAIGHDHKGHPHTSYYQYGLGILALCLHQKRVHDSVVDKLLYAVEPFHQGHHSVDTAAMAGLAFTCLKRSNFNPGRRQRITMAIRTVQEEILKAQTPEGHFGNVYSTPLALQFLMTSPMRGAELGTACLKARVALLASLQDGAFQNALMISQLLPVLNHKTYIDLIFPDCLAPRVMLEPAAETIPQTQEIISVTLQVLSLLPPYRQSISVLAGSTVEDVLKKAHELGGFTYETQASLSGPYLTSVMGKAAGEREFWQLLRDPNTPLLQGIADYRPKDGETIELRLVSW |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| E: ARG27 THR28 PHE29 LEU51 GLY52 VAL53 THR54 TYR56 ASN71 ALA72 TYR99 ASP100 ARG101 GLU102 B: PRO6 GLU7 MET8 ASP9 SER10 HIS11 SER44 ARG253 VAL254 LEU257 ASN279 HIS280 PRO294 ARG295 VAL296 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
