Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 465 |
| PDBID: | 3EO1 |
| Chains: | BA_C |
| Organism: | Mus musculus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.10 |
| Reference: | 10.1073/pnas.0807200106 |
| Antibody | |
| Antibody: | GC-1008 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Transforming growth factor beta-3 |
| Antigen mutation: | No |
| Durg Target: | P10600 |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >3EO1_B|Chain B, E, H, K|GC-1008 Fab Heavy Chain|Mus musculus (10090) QVQLVQSGAEVKKPGSSVKVSCKASGYTFSSNVISWVRQAPGQGLEWMGGVIPIVDIANYAQRFKGRVTITADESTSTTYMELSSLRSEDTAVYYCASTLGLVLDAMDYWGQGTLVTVSSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKRVESKYGPP |
Light Chain: A
Mutation: NULL
| >3EO1_A|Chain A, D, G, J|GC-1008 Fab Light Chain|Mus musculus (10090) ETVLTQSPGTLSLSPGERATLSCRASQSLGSSYLAWYQQKPGQAPRLLIYGASSRAPGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYADSPITFGQGTRLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: C
Mutation: NULL
| >3EO1_C|Chain C, F, I, L|Transforming growth factor beta-3|Homo sapiens (9606) ALDTNYCFRNLEENCCVRPLYIDFRQDLGWKWVHEPKGYYANFCSGPCPYLRSADTTHSTVLGLYNTLNPEASASPCCVPQDLEPLTILYYVGRTPKVEQLSNMVVKSCKCS |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: SER28 LEU29 GLY30 TYR33 TYR92 ALA93 ASP94 SER95 B: SER30 SER31 ASN32 VAL33 ILE52 PRO53 ILE54 VAL55 ILE57 ASN59 LEU100 GLY101 LEU102 VAL103 LEU104 C: LEU28 GLY29 TRP30 LYS31 TRP32 TYR90 TYR91 VAL92 GLY93 ARG94 THR95 GLU99 LEU101 MET104 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
