Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4638 |
| PDBID: | 7PRZ |
| Chains: | HL_E |
| Organism: | Homo sapiens, Severe acute respiratory syndrome coronavirus 2 |
| Method: | XRD |
| Resolution (Å): | 3.20 |
| Reference: | 10.1016/j.chom.2021.11.013 |
| Antibody | |
| Antibody: | beta-22 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | SARS-CoV-2 Beta variant spike glycoprotein RBD |
| Antigen mutation: | Yes |
| Durg Target: | P0DTC2 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7PRZ_H|Chain A[auth H]|Beta-22 Fab heavy chain|Homo sapiens (9606) EVQLVESGGGVVQPGRSLRLSCAASGFTFSNYGIHWVRQAPGKGLEWVAVISYDGSHKYYADSVKGRFTISRDNSKNTLYLQMNSLKTEDTAVYYCAKDSSAAIPYYYYGMDVWGQGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDK |
Light Chain: L
Mutation: NULL
| >7PRZ_L|Chain C[auth L]|Beta-22 Fab light chain|Homo sapiens (9606) AIQMTQSPDSLAVSLGERATINCKSSQSILYNSNNKTYLAWYQQKPGQPPKLLIFWASTRESGVPDRFSGSGSGTDFTLTISSLQAEDVAVYYCQQYYSIPLIFGPGTKVDIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: E
Mutation: K99N/E166K/N183Y
| >7PRZ_E|Chain B[auth E]|Spike protein S1|Severe acute respiratory syndrome coronavirus 2 (2697049) MGCVAETGHHHHHHTNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGNIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVKGFNCYFPLQSYGFQPTYGVGYQPYRVVVLSFELLHAPATVCGKK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: GLU1 VAL2 GLY26 PHE27 THR28 SER30 ASN31 TYR32 TYR53 ASP54 SER56 HIS57 LYS98 SER101 ALA102 ILE104 PRO105 TYR107 TYR109 ASP112 VAL113 L: ASN34 LYS36 TRP56 THR59 ARG60 SER62 E: ARG403 ASP405 ARG408 THR415 GLY416 ASN417 ASP420 TYR421 TYR449 TYR453 LEU455 PHE456 ARG457 ASN460 TYR473 ALA475 PHE486 ASN487 TYR489 PHE490 GLN493 SER494 TYR495 GLY496 THR500 TYR501 GLY502 VAL503 TYR505 GLN506 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
