Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4627 |
| PDBID: | 7PQG |
| Chains: | B_A |
| Organism: | Lama glama, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 3.70 |
| Reference: | 10.1038/s41586-022-04723-z |
| Antibody | |
| Antibody: | 87 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Human Na+–taurocholate co-transporting polypeptide (NTCP) EM |
| Antigen mutation: | Yes |
| Durg Target: | Q14973 |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >7PQG_B|Chain A[auth B]|Nanobody 87|Lama glama (9844) QVQLVESGGGLVQAGGSLRLSCAVSGRTTANYNMGWFRQAPGKEREFVAGIKWSSGSTYVADSAKGRFTISRDNAKNSVYLQMDSLKPEDTALYYCAANYYGVSWFLISPSSYDYWGQGTQVTVSSHHHHHHEPEA |
Antigen
Chain: A
Mutation: N4T/N10T/F32V/F36I/K85N/V94I/V106I/C128L/L220H
| >7PQG_A|Chain B[auth A]|Sodium/bile acid cotransporter|Homo sapiens (9606) MAHTASAPFTFTLPPNFGKRPTDLALSVILVVMLFIIMLSLGCTMEFSKIKAHLWKPKGLAIALVAQYGIMPLTAFVLGKVFRLNNIEALAILICGCSPGGNLSNIFSLAMKGDMNLSIVMTTCSTFLALGMMPLLLYIYSRGIYDGDLKDKVPYKGIVISLVLVLIPCTIGIVLKSKRPQYMRYVIKGGMIIILLCSVAVTVLSAINVGKSIMFAMTPHLIATSSLMPFIGFLLGYVLSALFCLNGRCRRTVSMETGCQNVQLCSTILNVAFPPEVIGPLFFFPLLYMIFQLGEGLLLIAIFWCYEKFKTPKDKTKMIYTAATTEELEVLFQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: GLN1 ARG27 THR29 ALA30 ASN31 THR58 TYR59 TYR100 TYR101 GLY102 VAL103 SER104 TRP105 PHE106 LEU107 ILE108 SER109 SER111 SER112 ASP114 TYR115 A: PHE12 THR13 LEU14 PRO15 PRO16 ASN86 ASN87 ILE88 GLU89 TYR146 ASP147 ASP149 LEU150 LYS151 ASP152 LYS153 PHE216 THR219 PHE274 PRO275 GLU277 VAL278 ILE279 GLY280 PRO281 LEU282 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
