Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4615 |
| PDBID: | 7PI7 |
| Chains: | BC_A |
| Organism: | Plasmodium falciparum 3D7, Gallus gallus |
| Method: | XRD |
| Resolution (Å): | 2.72 |
| Reference: | 10.1038/s41467-022-28601-4 |
| Antibody | |
| Antibody: | Cy.002 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Cysteine-rich protective antigen |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >7PI7_B|Chain B, E|Monoclonal antibody Cy.002 heavy chain|Gallus gallus (9031) EVQLQQSGAELVKPGASVKLSCTASGFNIKDTYIHWVKQRPEQGLEWIGRIDPANGNTYSDPKFQDKATIKADTSSNTAYLQLSSLTSEDTAVYYCARDVLYFDVWGAGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDK |
Light Chain: C
Mutation: NULL
| >7PI7_C|Chain C, F|Monoclonal antibody Cy.002 light chain|Gallus gallus (9031) DIVLTQSPASLAVSLGQRATISCRASESVDSYGNSFMHWYQQKPGQPPKLLISRASNLESGIPARFSGSGSRTDFTLTINPVEADDVATYYCQQSNEDRTFGGGTKLEIERTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: NULL
| >7PI7_A|Chain A, D|Cysteine-rich protective antigen|Plasmodium falciparum (isolate 3D7) (36329) DSRHVFIRTELSFIKNNVPCIRDMFFIYKRELYNICLDDLKGEEDETHIYVQKKVKDSWITLNDLFKETDLTGRPHIFAYVDVEEIIILLCEDEEFSNRKKDMTCHRFYSNDGKEYNNAEITISDYILKDKLLSSYVSLPLKIENREYFLICGVSPYKFKDDNKKDDILCMASHDKGETWGTKIVIKYDNYKLGVQYFFLRPYISKNDLSFHFYVGDNINNVKNVNFIECTHEKDLEFVCSNRDFLKDNKVLQDVSTLNDEYIVSYGNDNNFAECYIFFNNENSILIKPEKYGNTAAGCYGGTFVKIDENRALFIYSSSQGIYNIHTIYYANYEGGGGSEPEA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: PHE46 LYS49 ASP50 THR51 TYR52 HIS54 TRP66 ARG69 ASP71 ALA73 ASN74 ASN76 TYR78 ARG117 ASP118 VAL119 LEU120 TYR121 ASP123 C: SER51 TYR52 GLY53 ASN54 PHE56 ARG74 ASN77 GLU79 SER115 ASN116 GLU117 ASP118 ARG119 A: PHE124 SER125 LEU160 PRO184 TYR185 PHE187 LYS188 ASP189 TYR219 LYS220 LEU221 GLY222 VAL223 GLN224 VAL243 GLY244 ASP245 ASN246 ILE247 ASN248 VAL250 ASN252 LEU274 ASN298 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
