Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 460 |
| PDBID: | 3EHB |
| Chains: | CD_B |
| Organism: | Paracoccus denitrificans, Mus musculus |
| Method: | XRD |
| Resolution (Å): | 2.32 |
| Reference: | 10.1016/j.jmb.2008.09.074 |
| Antibody | |
| Antibody: | 3EHB Fv |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Cytochrome c oxidase |
| Antigen mutation: | No |
| Durg Target: | P08306 |
Sequence information
Antibody
Heavy Chain: C
Mutation: NULL
| >3EHB_C|Chain C|FV fragment Chain H|Mus musculus (10090) EVKLQESGGDLVQPGGSLKLSCAASGFTFSSYTMSWVRQTPEKRLEWVASINNGGGRTYYPDTVKGRFTISRDNAKNTLYLQMSSLKSEDTAMYYCVRHEYYYAMDYWGQGTTVTVSSAWRHPQFGG |
Light Chain: D
Mutation: NULL
| >3EHB_D|Chain D|FV fragment Chain L|Mus musculus (10090) DIELTQTPVSLSASVGETVTITCRASENIYSYLAWYQQKQGKSPQFLVYNAKTLGEGVPSRFSGSGSGTQFSLKINSLLPEDFGSYYCQHHYGTPPLTFGGGTKLEIKREQKLISEEDLM |
Antigen
Chain: B
Mutation: NULL
| >3EHB_B|Chain B|Cytochrome c oxidase subunit 2|Paracoccus denitrificans (266) MMAIATKRRGVAAVMSLGVATMTAVPALAQDVLGDLPVIGKPVNGGMNFQPASSPLAHDQQWLDHFVLYIITAVTIFVCLLLLICIVRFNRRANPVPARFTHNTPIEVIWTLVPVLILVAIGAFSLPILFRSQEMPNDPDLVIKAIGHQWYWSYEYPNDGVAFDALMLEKEALADAGYSEDEYLLATDNPVVVPVGKKVLVQVTATDVIHAWTIPAFAVKQDAVPGRIAQLWFSVDQEGVYFGQCSELCGINHAYMPIVVKAVSQEKYEAWLAGAKEEFAADASDYLPASPVKLASAE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| C: GLU1 THR28 SER31 TYR32 ASN53 ARG57 ARG98 GLU100 TYR101 TYR102 D: ASN28 TYR30 SER31 TYR32 TYR49 ASN50 LYS52 THR53 GLU56 TYR92 B: ALA23 PRO26 HIS29 ASP30 TRP33 ASP109 ASP111 VAL166 GLY167 LYS168 TRP203 SER205 ASP207 GLN208 GLU209 SER235 GLN236 GLU237 LYS238 GLU240 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
