Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4593 |
| PDBID: | 7PHU |
| Chains: | BC_A |
| Organism: | Plasmodium falciparum 3D7, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.53 |
| Reference: | 10.1038/s41467-022-28601-4 |
| Antibody | |
| Antibody: | R5.015 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Reticulocyte-binding protein homolog 5 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: B
Mutation: NULL
| >7PHU_B|Chain B|Monoclonal antibody R5.015 heavy chain|Homo sapiens (9606) VHSEVQLVQSGAEVKKPGASVKVSCKASGYTFTDYYIHWVRQAPGQGLEWMGWINPNSGGTNYAQKFQGWVTMTRDTSISTTYMELSRLRSDDMAVYYCARDLWFGESPPYYGVDVWGQGTTVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTC |
Light Chain: C
Mutation: NULL
| >7PHU_C|Chain C|Monoclonal antibody R5.015 light chain|Homo sapiens (9606) SVTSYELTQPPSVSVAPGKTAKITCGGNNIGSYSVHWYQQKPGQAPVLVIYYDSDRPSGIPERFSGSNSGNTATLTISRVEVGDEADYFCQVWDTNTDHVVFGGGTKLTVLGQPKAAPSVTLFPPSSEELQANKATLVCLISDFYPGAVTVAWKADSSPVKAGVETTTPSKQSNNKYAASSYLSLTPEQWKSHRSYSCQVTHEGSTVEKTVAPTECS |
Antigen
Chain: A
Mutation: NULL
| >7PHU_A|Chain A|Reticulocyte-binding protein homolog 5|Plasmodium falciparum (isolate 3D7) (36329) ENAIKKTKNQENQLTLLPIKSTEEEKDDIKNGKDIKKEIDNDKENIKTNNAKDHSTYIKSYLNTNVNDGLKYLFIPSHNSFIKKYSVFNQINDGMLLNEKNDVKNNEDYKNVDYKNVNFLQYHFKELSNYNIANSIDILQEKEGHLDFVIIPHYTFLDYYKHLSYNSIYHKSSTYGKYIAVDAFIKKINEAYDKVKSKCNDIKNDLIATIKKLEHPYDINNKNDDSYRYDISEEIDDKSEETDDETEEVEDSIQDTDSNHTPSNKKKNDLMNRTFKKMMDEYNTKKKKLIKCIKNHENDFNKICMDMKNYGTNLFEQLSCYNNNFCNTNGIRYHYDEYIHKLILSVKSKNLNKDLSDMTNILQQSELLLTNLNKKMGSYIYIDTIKFIHKEMKHIFNRIEYHTKIINDKTKIIQDKIKLNIWRTFQKDELLKRILDMSNEYSLFITSDHLRQMLYNTFYSKEKHLNNIFHHLIYVLQMKFNDVPIKMEYFQTYKKNKPLTQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: THR33 ASP36 TYR37 LEU105 TRP106 PHE107 GLU109 SER110 PRO111 PRO112 TYR113 TYR114 C: ASN25 ASN26 GLY28 SER29 TYR30 SER31 TYR48 TYR49 ASP50 SER51 ASP52 ASN65 GLY67 ASN68 TRP90 ASP91 THR92 ASN93 A: LYS378 ASP382 ASN385 ILE386 GLN389 SER390 LEU392 LEU393 ASN396 LEU397 LYS399 LYS400 GLU487 LYS488 ASN491 PHE494 HIS495 ILE498 TYR499 LEU501 GLN502 MET503 LYS504 PHE505 ASN506 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
