Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4590 |
| PDBID: | 7PHP |
| Chains: | N_A |
| Organism: | Vibrio cholerae RC385, synthetic construct, Lama glama |
| Method: | EM |
| Resolution (Å): | 3.47 |
| Reference: | 10.1073/pnas.2115435118 |
| Antibody | |
| Antibody: | NorM-Nb17_4 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | bacterial MATE transporter VcNorM |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: N
Mutation: NULL
| >7PHP_N|Chain D[auth N]|NorM-Nb17_4|synthetic construct (32630) QRQLVESGGGLVQPGGSLRLSCAASGIIFKINDMGWFRQAPGKEREGVAGITSGGRTNYADSVKGRFIISRDNVKNTVYLQMNSLEPEDTAVYYCKSDGLISYAASQLSTYWGKGTPVTVSSHHHHHHEPEA |
Antigen
Chain: A
Mutation: NULL
| >7PHP_A|Chain A|Multidrug resistance protein NorM|Vibrio cholerae RC385 (345074) MGHHHHHHHHHHSSGLEVLFQGPSGTSVHRYKKEASNLIKLATPVLIASVAQTGMGFVDTIMAGGVSAIDMAAVSIAASIWLPSILFGVGLLMALVPVVAQLNGAGRQHKIPFEVHQGLILALLVSIPIIAVLFQTQFIIRFMDVEEAMATKTVGYMHAVIFAVPAYLLFQALRSFTDGMSLTKPAMVIGFIGLLLNIPLNWIFVYGKFGAPELGGVGCGVATAIVYWIMLLLLLFYIVTSKRLAHVKVFETFHKPQPKELIRLFRLGFPVAAALFFEVTLFAVVALLVAPLGSTVVAAHQVALNFSSLVFMFPMSIGAAVSIRVGHKLGEQDTKGAAIAANVGLMTGLATACITALLTVLFREQIALLYTENQVVVALAMQLLLFAAIYQCMDAVQVVAAGSLRGYKDMTAIFHRTFISYWVLGLPTGYILGMTNWLTEQPLGAKGFWLGFIIGLSAAALMLGQRLYWLQKQSDDVQLHLAAK |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| N: ARG2 GLN3 GLY26 ILE27 ILE28 LYS30 ILE31 ASP33 THR52 SER53 GLY54 ASP95 GLY96 LEU97 ILE98 SER99 TYR102 A: PHE30 THR33 ILE34 GLY37 GLY38 VAL39 SER40 ALA41 ILE42 MET44 ALA45 SER48 ILE49 SER52 MET116 ASP117 VAL118 MET122 SER267 THR268 ALA271 GLN274 VAL275 ASN278 LEU342 TYR343 THR344 GLU345 ASN346 GLN347 VAL348 VAL349 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
