Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4565 |
| PDBID: | 7P6K |
| Chains: | H_D |
| Organism: | Mus musculus, synthetic construct |
| Method: | EM |
| Resolution (Å): | 3.80 |
| Reference: | 10.1038/s41467-021-25742-w |
| Antibody | |
| Antibody: | Sb5 synthetic-Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Volume-regulated anion channel subunit LRRC8A |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: H
Mutation: NULL
| >7P6K_H|Chain G, H, I|synthetic nanobody Sb5|synthetic construct (32630) GSSSQVQLVESGGGLVQAGGSLRLSCAASGFPVAQEIMTWYRQAPGKEREWVAAISSIGDTTAYADSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCAVNVGFTYKGQGTQVTVSAGRAGEQKLISEEDLNSAVDHHHHHH |
Antigen
Chain: D
Mutation: NULL
| >7P6K_D|Chain A, B, C, D, E, F|Volume-regulated anion channel subunit LRRC8A|Mus musculus (10090) MIPVTELRYFADTQPAYRILKPWWDVFTDYISIVMLMIAVFGGTLQVTQDKMICLPCKWVTKDSCNDSFRGWAASSPEPTYPNSTVLPTPDTGPTGIKYDLDRHQYNYVDAVCYENRLHWFAKYFPYLVLLHTLIFLACSNFWFKFPRTSSKLEHFVSILLKCFDSPWTTRALSETVVEESDPKPAFSKMNGSMDKKSSTVSEDVEATVPMLQRTKSRIEQGIVDRSETGVLDKKEGEQAKALFEKVKKFRTHVEEGDIVYRLYMRQTIIKVIKFALIICYTVYYVHNIKFDVDCTVDIESLTGYRTYRCAHPLATLFKILASFYISLVIFYGLICMYTLWWMLRRSLKKYSFESIREESSYSDIPDVKNDFAFMLHLIDQYDPLYSKRFAVFLSEVSENKLRQLNLNNEWTLDKLRQRLTKNAQDKLELHLFMLSGIPDTVFDLVELEVLKLELIPDVTIPPSIAQLTGLKELWLYHTAAKIEAPALAFLRENLRALHIKFTDIKEIPLWIYSLKTLEELHLTGNLSAENNRYIVIDGLRELKRLKVLRLKSNLSKLPQVVTDVGVHLQKLSINNEGTKLIVLNSLKKMVNLTELELIRCDLERIPHSIFSLHNLQEIDLKDNNLKTIEEIISFQHLHRLTCLKLWYNHIAYIPIQIGNLTNLERLYLNRNKIEKIPTQLFYCRKLRYLDLSHNNLTFLPADIGLLQNLQNLAVTANRIEALPPELFQCRKLRALHLGNNVLQSLPSRVGELTNLTQIELRGNRLECLPVELGECPLLKRSGLVVEEDLFSTLPPEVKERLWRADKEQA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition H: PHE27 PRO28 VAL29 GLN31 GLU32 ILE33 THR35 TRP47 ALA50 ILE51 SER52 SER53 ILE54 THR57 THR58 ALA59 ASN99 VAL100 GLY101 D: ARG419 HIS431 PHE433 MET434 LYS452 GLU454 LEU455 TRP475 TYR477 HIS478 LYS501 THR503 THR524 ASN526 LYS552 GLU577 ARG600 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
