Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4562 |
| PDBID: | 7P60 |
| Chains: | H_D |
| Organism: | Mus musculus, synthetic construct |
| Method: | EM |
| Resolution (Å): | 3.80 |
| Reference: | 10.1038/s41467-021-25742-w |
| Antibody | |
| Antibody: | Sb4 synthetic-Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Volume-regulated anion channel subunit LRRC8A |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: H
Mutation: NULL
| >7P60_H|Chain G, H, I|synthetic nanobody Sb4|synthetic construct (32630) GSSSQVQLVESGGGSVQAGGSLRLSCAASGYIYQIEYLGWFRQAPGKEREGVAALATWNGQTYYADSVKGRFTVSLDNAKNTVYLQMNSLKPEDTALYYCAAAYEGDTSPLYYEEYGYWGQGTQVTVSAGRAGEQKLISEEDLNSAVDHHHHHH |
Antigen
Chain: D
Mutation: NULL
| >7P60_D|Chain A, B, C, D, E, F|Volume-regulated anion channel subunit LRRC8A|Mus musculus (10090) MIPVTELRYFADTQPAYRILKPWWDVFTDYISIVMLMIAVFGGTLQVTQDKMICLPCKWVTKDSCNDSFRGWAASSPEPTYPNSTVLPTPDTGPTGIKYDLDRHQYNYVDAVCYENRLHWFAKYFPYLVLLHTLIFLACSNFWFKFPRTSSKLEHFVSILLKCFDSPWTTRALSETVVEESDPKPAFSKMNGSMDKKSSTVSEDVEATVPMLQRTKSRIEQGIVDRSETGVLDKKEGEQAKALFEKVKKFRTHVEEGDIVYRLYMRQTIIKVIKFALIICYTVYYVHNIKFDVDCTVDIESLTGYRTYRCAHPLATLFKILASFYISLVIFYGLICMYTLWWMLRRSLKKYSFESIREESSYSDIPDVKNDFAFMLHLIDQYDPLYSKRFAVFLSEVSENKLRQLNLNNEWTLDKLRQRLTKNAQDKLELHLFMLSGIPDTVFDLVELEVLKLELIPDVTIPPSIAQLTGLKELWLYHTAAKIEAPALAFLRENLRALHIKFTDIKEIPLWIYSLKTLEELHLTGNLSAENNRYIVIDGLRELKRLKVLRLKSNLSKLPQVVTDVGVHLQKLSINNEGTKLIVLNSLKKMVNLTELELIRCDLERIPHSIFSLHNLQEIDLKDNNLKTIEEIISFQHLHRLTCLKLWYNHIAYIPIQIGNLTNLERLYLNRNKIEKIPTQLFYCRKLRYLDLSHNNLTFLPADIGLLQNLQNLAVTANRIEALPPELFQCRKLRALHLGNNVLQSLPSRVGELTNLTQIELRGNRLECLPVELGECPLLKRSGLVVEEDLFSTLPPEVKERLWRADKEQA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition H: ILE28 TYR29 GLU32 TYR33 GLY42 GLU44 ARG45 ALA52 TRP54 ASN55 GLN57 TYR59 GLU101 GLY102 THR104 SER105 PRO106 TYR108 TYR109 GLU110 GLU111 D: HIS431 PHE433 MET434 GLU454 LEU455 TRP475 TYR477 HIS478 LYS501 THR503 THR524 GLY525 ASN526 ARG550 LYS552 ASN554 ASN575 GLU577 ILE599 ARG600 LYS622 ASP623 TRP647 TYR648 ASN670 ARG671 SER693 HIS694 THR716 ALA717 ARG719 ASN740 ARG765 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
