Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4549 |
| PDBID: | 7P5W |
| Chains: | G_A |
| Organism: | Mus musculus, synthetic construct |
| Method: | EM |
| Resolution (Å): | 3.50 |
| Reference: | 10.1038/s41467-021-25742-w |
| Antibody | |
| Antibody: | Sb2 synthetic-Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Volume-regulated anion channel subunit LRRC8A |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: G
Mutation: NULL
| >7P5W_G|Chain G, H, I, J, K, L|synthetic nanobody Sb2|synthetic construct (32630) GSSSQVQLVESGGGLVQAGGSLRLSCAASGFPVYQAWMWWYRQAPGKEREWVAAIESEGQYTWYADSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCNVKDTGHTTNQYDYWGQGTQVTVSAGRAGEQKLISEEDLNSAVDHHHHHH |
Antigen
Chain: A
Mutation: NULL
| >7P5W_A|Chain A, B, C, D, E, F|Volume-regulated anion channel subunit LRRC8A|Mus musculus (10090) MIPVTELRYFADTQPAYRILKPWWDVFTDYISIVMLMIAVFGGTLQVTQDKMICLPCKWVTKDSCNDSFRGWAASSPEPTYPNSTVLPTPDTGPTGIKYDLDRHQYNYVDAVCYENRLHWFAKYFPYLVLLHTLIFLACSNFWFKFPRTSSKLEHFVSILLKCFDSPWTTRALSETVVEESDPKPAFSKMNGSMDKKSSTVSEDVEATVPMLQRTKSRIEQGIVDRSETGVLDKKEGEQAKALFEKVKKFRTHVEEGDIVYRLYMRQTIIKVIKFALIICYTVYYVHNIKFDVDCTVDIESLTGYRTYRCAHPLATLFKILASFYISLVIFYGLICMYTLWWMLRRSLKKYSFESIREESSYSDIPDVKNDFAFMLHLIDQYDPLYSKRFAVFLSEVSENKLRQLNLNNEWTLDKLRQRLTKNAQDKLELHLFMLSGIPDTVFDLVELEVLKLELIPDVTIPPSIAQLTGLKELWLYHTAAKIEAPALAFLRENLRALHIKFTDIKEIPLWIYSLKTLEELHLTGNLSAENNRYIVIDGLRELKRLKVLRLKSNLSKLPQVVTDVGVHLQKLSINNEGTKLIVLNSLKKMVNLTELELIRCDLERIPHSIFSLHNLQEIDLKDNNLKTIEEIISFQHLHRLTCLKLWYNHIAYIPIQIGNLTNLERLYLNRNKIEKIPTQLFYCRKLRYLDLSHNNLTFLPADIGLLQNLQNLAVTANRIEALPPELFQCRKLRALHLGNNVLQSLPSRVGELTNLTQIELRGNRLECLPVELGECPLLKRSGLVVEEDLFSTLPPEVKERLWRADKEQA |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition G: GLN31 TRP33 TRP35 TYR37 GLU44 ARG45 GLU46 TRP47 GLU52 SER53 GLU54 TYR57 TRP59 TYR60 ALA61 ASP62 LYS99 ASP100 THR101 HIS103 THR104 THR105 ASN106 GLN107 TYR108 ASP109 TRP111 A: LYS482 ILE483 ALA485 LEU488 ALA489 ARG492 LEU510 TRP511 TYR513 SER514 LYS516 GLU530 ASN531 ASN532 ARG533 ILE535 ASP538 GLY539 ARG541 GLU542 LYS544 GLN560 ASP564 VAL567 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
