Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4516 |
| PDBID: | 7OH1 |
| Chains: | ED_A |
| Organism: | Clostridium tetani, Homo sapiens |
| Method: | EM |
| Resolution (Å): | 8.00 |
| Reference: | 10.1172/JCI151676 |
| Antibody | |
| Antibody: | TT110 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Tetanus neurotoxin |
| Antigen mutation: | No |
| Durg Target: | P04958 |
Sequence information
Antibody
Heavy Chain: E
Mutation: NULL
| >7OH1_E|Chain B[auth E]|FAB TT110|Homo sapiens (9606) QVQLMQSGAEVQKPGASVKVSCQASGFTLNNYYIHWLRQAPGQGFEWMGIFNPSSGTRTYAQKFQGRISMTADASTTTLYMELSGLTSEDAGVYFCARIGGSTYGRLMTYYFDHWGQGTVVAVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSC |
Light Chain: D
Mutation: NULL
| >7OH1_D|Chain C[auth D]|FAB TT110|Homo sapiens (9606) VLTQGPVTLSVSPGGRGTLSCRASRSISTTLAWYQQKPGQAPRLLIYGASTRATGIPARFTGSGSGTEFTLTISSLQSEDFAVYYCQQYNDWPVTFGQGTQVEVKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: A
Mutation: NULL
| >7OH1_A|Chain A|Tetanus toxin|Clostridium tetani (1513) MPITINNFRYSDPVNNDTIIMMEPPYCKGLDIYYKAFKITDRIWIVPERYEFGTKPEDFNPPSSLIEGASEYYDPNYLRTDSDKDRFLQTMVKLFNRIKNNVAGEALLDKIINAIPYLGNSYSLLDKFDTNSNSVSFNLLEQDPSGATTKSAMLTNLIIFGPGPVLNKNEVRGIVLRVDNKNYFPCRDGFGSIMQMAFCPEYVPTFDNVIENITSLTIGKSKYFQDPALLLMHELIHVLHGLYGMQVSSHEIIPSKQEIYMQHTYPISAEELFTFGGQDANLISIDIKNDLYEKTLNDYKAIANKLSQVTSCNDPNIDIDSYKQIYQQKYQFDKDSNGQYIVNEDKFQILYNSIMYGFTEIELGKKFNIKTRLSYFSMNHDPVKIPNLLDDTIYNDTEGFNIESKDLKSEYKGQNMRVNTNAFRNVDGSGLVSKLIGLCKKIIPPTNIRENLYNRTASLTDLGGELCIKIKNEDLTFIAEKNSFSEEPFQDEIVSYNTKNKPLNFNYSLDKIIVDYNLQSKITLPNDRTTPVTKGIPYAPEYKSNAASTIEIHNIDDNTIYQYLYAQKSPTTLQRITMTNSVDDALINSTKIYSYFPSVISKVNQGAQGILFLQWVRDIIDDFTNESSQKTTIDKISDVSTIVPYIGPALNIVKQGYEGNFIGALETTGVVLLLEYIPEITLPVIAALSIAESSTQKEKIIKTIDNFLEKRYEKWIEVYKLVKAKWLGTVNTQFQKRSYQMYRSLEYQVDAIKKIIDYEYKIYSGPDKEQIADEINNLKNKLEEKANKAMININIFMRESSRSFLVNQMINEAKKQLLEFDTQSKNILMQYIKANSKFIGITELKKLESKINKVFSTPIPFSYSKNLDCW |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition E: TYR33 ILE50 ASN52 PRO53 SER54 SER55 GLY56 THR57 ARG58 THR59 TYR60 GLN65 GLY66 THR71 ALA72 TYR104 GLY105 ARG106 LEU107 MET108 THR109 TYR110 D: ARG255 SER256 ILE257 SER258 THR259 THR260 GLY296 ASP321 TRP322 A: THR579 ASN580 TYR593 SER594 TYR595 PRO597 SER598 ILE600 SER601 LYS602 ASN604 GLN605 GLN614 ARG617 ASP618 ASP621 ASP622 THR624 ASN625 GLU626 SER628 GLN629 LYS630 THR631 PRO644 PRO648 GLY656 TYR657 GLU658 GLY659 ASN660 ILE662 GLY663 LYS788 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
