Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4514 |
| PDBID: | 7OCJ |
| Chains: | B_D |
| Organism: | Lama glama, Escherichia coli |
| Method: | XRD |
| Resolution (Å): | 2.70 |
| Reference: | 10.1016/j.str.2022.09.004 |
| Antibody | |
| Antibody: | NbSOS2 (14509) Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Escherichia coli lexA repressor |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >7OCJ_B|Chain A[auth B], D[auth E], E[auth G], H|NbSOS2 (14509)|Lama glama (9844) QVQLVESGGGLVQAGGSLRLSCAASGSIRSLNAMGWYRQAPGKQRELVAAITSRGSTRYGDFVKGRFTISRGNAKNTVYLQMNSLSVEDTAVYYCKQTQLGYDYWGQGTQVTVSS |
Antigen
Chain: D
Mutation: NULL
| >7OCJ_D|Chain B[auth A], C, F[auth D], G[auth F]|LexA repressor|Escherichia coli (562) MGSSHHHHHHSSGLVPRGSHMKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEEHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| B: SER30 LEU31 ASN32 ALA33 THR52 SER53 ARG54 SER56 LYS96 THR98 LEU100 GLY101 ASP103 D: GLU93 GLU94 ASP121 PRO122 SER123 LEU124 LYS126 PRO127 ASN128 HIS154 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
