Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4513 |
| PDBID: | 7OCJ |
| Chains: | G_C |
| Organism: | Lama glama, Escherichia coli |
| Method: | XRD |
| Resolution (Å): | 2.70 |
| Reference: | 10.1016/j.str.2022.09.004 |
| Antibody | |
| Antibody: | NbSOS2 (14509) Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Escherichia coli lexA repressor |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: G
Mutation: NULL
| >7OCJ_G|Chain A[auth B], D[auth E], E[auth G], H|NbSOS2 (14509)|Lama glama (9844) QVQLVESGGGLVQAGGSLRLSCAASGSIRSLNAMGWYRQAPGKQRELVAAITSRGSTRYGDFVKGRFTISRGNAKNTVYLQMNSLSVEDTAVYYCKQTQLGYDYWGQGTQVTVSS |
Antigen
Chain: C
Mutation: NULL
| >7OCJ_C|Chain B[auth A], C, F[auth D], G[auth F]|LexA repressor|Escherichia coli (562) MGSSHHHHHHSSGLVPRGSHMKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEEHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition G: ALA33 MET34 GLY35 TYR37 ALA50 ILE51 THR52 ARG54 GLY55 SER56 THR57 ARG58 TYR59 GLY60 ASP61 LYS64 GLY65 THR68 LYS96 THR98 C: GLU93 GLU94 LEU98 ARG101 GLN111 GLN112 ILE114 GLU115 GLY116 HIS117 TYR118 GLN119 ARG134 GLY219 ASP220 TRP221 LEU222 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
