Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4504 |
| PDBID: | 7O52 |
| Chains: | HL_U |
| Organism: | Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 2.41 |
| Reference: | 10.1016/j.jbc.2021.100966 |
| Antibody | |
| Antibody: | m971 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | CD22 d6-d7 Ig domains |
| Antigen mutation: | No |
| Durg Target: | P20273 |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7O52_H|Chain A[auth H]|m971 Fab Heavy chain|Homo sapiens (9606) TGQVQLQQSGPGLVKPSQTLSLTCAISGDSVSSNSAAWNWIRQSPSRGLEWLGRTYYRSKWYNDYAVSVKSRITINPDTSKNQFSLQLNSVTPEDTAVYYCAREVTGDLEDAFDIWGQGTMVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVSHKPSNTKVDKKVEPKSC |
Light Chain: L
Mutation: NULL
| >7O52_L|Chain B[auth L]|m971 Fab Light chain|Homo sapiens (9606) TGDIQMTQSPSSLSASVGDRVTITCRASQTIWSYLNWYQQRPGKAPNLLIYAASSLQSGVPSRFSGRGSGTDFTLTISSLQAEDFATYYCQQSYSIPQTFGQGTKLEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: U
Mutation: NULL
| >7O52_U|Chain C[auth U]|CD22 d6-d7 Ig domains|Homo sapiens (9606) TGPRDVRVRKIKPLSEIHSGNSVSLQCDFSSSHPKEVQFFWEKNGRLLGKESQLNFDSISPEDAGSYSCWVNNSIGQTASKAWTLEVLYAPRRLRVSMSPGDQVMEGKSATLTCESDANPPVSHYTWFDWNNQSLPYHSQKLRLEPVKVQHSGAYWCQGTNSVGKGRSPLSTLTVYYSPETIGRRGTKHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| H: ASN32 ARG50 ARG52 SER53 LYS54 TRP55 TYR56 ASN57 ASP58 TYR59 VAL61 LYS64 THR97 GLY98 ASP99 L: TRP30 TYR32 SER91 TYR92 SER93 ILE94 U: LYS610 PRO623 VAL624 SER625 HIS626 TYR627 THR628 ASP631 TRP632 ASN633 GLN635 SER636 LEU637 PRO638 TYR639 HIS640 SER641 GLN642 ARG645 LEU646 GLU647 PRO648 LYS650 GLN652 HIS653 TYR657 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
