Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4500 |
| PDBID: | 7O0S |
| Chains: | A_B |
| Organism: | Camelidae mixed library, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 1.70 |
| Reference: | 10.1016/j.str.2021.08.007 |
| Antibody | |
| Antibody: | 36Z Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | N-terminal domain of centrosomal protein of 164 kDa |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: A
Mutation: NULL
| >7O0S_A|Chain A|Nanobody 36Z|Camelidae mixed library (1579311) GPLGSMGQVQLQESGGGLVQAGGSLNLSCVASGSSHFNSMGWYRQAPGKQRDLVADISNDGVTNYADSVKDRFTISTNNAKNAVYLQMNNLKPEDTAVYYCNAVAVAGRAFSYWGQGTQVTVSSAA |
Antigen
Chain: B
Mutation: NULL
| >7O0S_B|Chain B|Centrosomal protein of 164 kDa|Homo sapiens (9606) GPLGSMAGRPLRIGDQLVLEEDYDETYIPSEQEILEFAREIGIDPIKEPELMWLAREGIVAPLPGEWKPCQDITGDIYYFNFANGQSMWDHPCDEHYRSLVIQERAKLSTSGAI |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| A: GLN3 VAL4 SER30 HIS31 PHE32 ASN33 SER34 TYR38 GLN45 ARG46 ASP47 LEU48 ASP51 ILE52 SER53 ASP55 VAL57 THR58 ASN59 ASN97 VAL99 ALA100 VAL101 ALA102 GLY103 ARG104 ALA105 PHE106 SER107 TYR108 TRP109 B: ALA2 GLY3 ARG4 LEU6 ARG7 LEU12 LEU14 GLU15 GLU16 GLU20 THR21 TYR22 PRO24 SER25 GLN27 GLU28 PHE32 GLY53 ILE54 VAL55 ALA56 PRO57 LEU58 PRO64 GLN66 ILE68 THR69 GLY70 ASP71 ILE72 TYR74 HIS86 ASP89 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
