Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 450 |
| PDBID: | 3CVH |
| Chains: | QR_M |
| Organism: | Mus musculus, Gallus gallus |
| Method: | XRD |
| Resolution (Å): | 2.90 |
| Reference: | 10.1074/jbc.M804996200 |
| Antibody | |
| Antibody: | 25-D1.16 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | H-2 class I histocompatibility antigen, K-B alpha chain |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: Q
Mutation: NULL
| >3CVH_Q|Chain D[auth H], I[auth Q]|25-D1.16 heavy chain|Mus musculus (10090) VLLQQSGPELVKPGASVKIPCKASGYTFTDYNMDWVKQSHGKSLEWIGDINPNNGGTIYNQKFKGKATLTVDKSSSAAYMEVRSLTSEDTAVYYCARKPYYGNFAWFAYWGQGTLVTVSAAKTTPPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIVP |
Light Chain: R
Mutation: NULL
| >3CVH_R|Chain E[auth L], J[auth R]|25-D1.16 light chain|Mus musculus (10090) IQVTQSSSSFSVSLGDRVTITCKASEDIYNRLAWYQQKPGNAPRLLISGATSLETGVPDRFSGSGSRKDYTLIITSLQTEDVATYYCQQYWSTPLTFGAGTKLELKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFN |
Antigen
Chain: M
Mutation: NULL
| >3CVH_M|Chain A, F[auth M]|H-2 class I histocompatibility antigen, K-B alpha chain|Mus musculus (10090) GPHSLRYFVTAVSRPGLGEPRYMEVGYVDDTEFVRFDSDAENPRYEPRARWMEQEGPEYWERETQKAKGNEQSFRVDLRTLLGYYNQSKGGSHTIQVISGCEVGSDGRLLRGYQQYAYDGCDYIALNEDLKTWTAADMAALITKHKWEQAGEAERLRAYLEGTCVEWLRRYLKNGNATLLRTDSPKAHVTHHSRPEDKVTLRCWALGFYPADITLTWQLNGEELIQDMELVETRPAGDGTFQKWASVVVPLGKEQYYTCHVYHQGLPEPLTLRW |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Q: ASP30 ASN32 ASN51 ASN53 ASN54 GLY55 GLY56 THR57 ILE58 TYR59 GLN61 LYS64 TYR100 TYR101 GLY102 ASN103 PHE104 R: GLU26 ASP27 TYR29 ARG31 TRP91 SER92 THR93 M: ARG62 GLN65 LYS66 LYS68 GLY69 GLN72 SER73 ARG75 VAL76 ASP77 ARG79 THR80 LYS89 LYS146 GLN149 ALA150 GLY151 GLU152 GLU154 ARG155 ALA158 TYR159 GLY162 THR163 GLU166 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
