Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4499 |
| PDBID: | 7O06 |
| Chains: | B_D |
| Organism: | Camelidae mixed library, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 1.60 |
| Reference: | 10.1016/j.str.2021.08.007 |
| Antibody | |
| Antibody: | 10Z Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | N-terminal domain of centrosomal protein of 164 kDa |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >7O06_B|Chain A, B|Camelid nanobody 10Z|Camelidae mixed library (1579311) GPLGSMGQVQLQESGGGLVQPGGSLRLSCSASGSSFSINTMGWYRQALGKQRELVANINSGGSTNYIDSVKGRFTISRDNAKNMVYLQMNSLKPEDTAVYFCNAARPLRPEGGRWLNYWGQGTQVTVSSAA |
Antigen
Chain: D
Mutation: NULL
| >7O06_D|Chain C, D|Centrosomal protein of 164 kDa|Homo sapiens (9606) GPLGSMAGRPLRIGDQLVLEEDYDETYIPSEQEILEFAREIGIDPIKEPELMWLAREGIVAPLPGEWKPCQDITGDIYYFNFANGQSMWDHPCDEHYRSLVIQERAKLSTSGAI |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition B: GLN8 VAL9 SER34 SER35 SER37 ILE38 THR40 TYR44 GLN51 ARG52 GLU53 LEU54 ASN57 ASN59 SER60 GLY61 SER63 ASN65 TYR66 ILE67 ASN103 ALA105 ARG106 PRO107 LEU108 ARG109 PRO110 GLU111 GLY112 GLY113 ARG114 TRP115 LEU116 ASN117 TYR118 TRP119 D: ARG4 LEU6 ARG7 ILE8 GLY9 ASP19 THR21 TYR22 ILE23 PRO24 SER25 GLN27 GLU28 PHE32 GLU35 GLY53 ILE54 VAL55 ALA56 PRO57 LEU58 TRP62 LYS63 PRO64 GLN66 ASP67 ILE68 THR69 GLY70 ASP71 ILE72 TYR74 HIS86 ASP89 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
