Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4483 |
| PDBID: | 7NVO |
| Chains: | N_E |
| Organism: | Homo sapiens, Lama glama |
| Method: | EM |
| Resolution (Å): | 3.50 |
| Reference: | 10.1038/s41594-022-00755-1 |
| Antibody | |
| Antibody: | Nb18 VHH |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | T-complex protein 1 subunit epsilon |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: N
Mutation: NULL
| >7NVO_n|Chain I[auth N], R[auth n]|Nanobody|Lama glama (9844) QVQLVESGGGLVQAGGSLRLSCGASGTFFRINDMGWYRQASGKQRELVASITRGGTTDYADSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYYCKANRNWGREWDDYWGQGTQVTVSSHHHHHHEPEA |
Antigen
Chain: E
Mutation: NULL
| >7NVO_e|Chain A[auth E], J[auth e]|T-complex protein 1 subunit epsilon|Homo sapiens (9606) MASMGTLAFDEYGRPFLIIKDQDRKSRLMGLEALKSHIMAAKAVANTMRTSLGPNGLDKMMVDKDGDVTVTNDGATILSMMDVDHQIAKLMVELSKSQDDEIGDGTTGVVVLAGALLEEAEQLLDRGIHPIRIADGYEQAARVAIEHLDKISDSVLVDIKDTEPLIQTAKTTLGSKVVNSCHRQMAEIAVNAVLTVADMERRDVDFELIKVEGKVGGRLEDTKLIKGVIVDKDFSHPQMPKKVEDAKIAILTCPFEPPKPKTKHKLDVTSVEDYKALQKYEKEKFEEMIQQIKETGANLAICQWGFDDEANHLLLQNNLPAVRWVGGPEIELIAIATGGRIVPRFSELTAEKLGFAGLVQEISFGTTKDKMLVIEQCKNSRAVTIFIRGGNKMIIEEAKRSLHDALCVIRNLIRDNRVVYGGGAAEISCALAVSQEADKCPTLEQYAMRAFADALEVIPMALSENSGMNPIQTMTEVRARQVKEMNPALGIDCLHKGTNDMKQQHVIETLIGKKQQISLATQMVRMILKIDDIRKPGESEE |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| n: THR27 PHE29 ARG30 ASN100 TRP101 ARG103 TRP105 e: HIS147 LYS150 ILE151 ASP153 SER154 TYR420 GLU484 MET485 ASN486 PRO487 ALA488 LYS502 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
