Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4475 |
| PDBID: | 7NRH |
| Chains: | HL_A |
| Organism: | Oryctolagus cuniculus, Orthohantavirus hantanense |
| Method: | EM |
| Resolution (Å): | 19.00 |
| Reference: | 10.1128/mBio.02531-20 |
| Antibody | |
| Antibody: | HTN-Gn1 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Hantaan virus glycoprotein |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7NRH_H|Chain A[auth H]|Fab fragment HTN-Gn1 Heavy chain|Oryctolagus cuniculus (9986) TGQSLVESGGDLVKPEGSLTLTCTASGFSFSSTHWICWVRQAPGKGLEWIACIYVGNTYDSYYANWAKGRFTISKTSSTTVTLQMTTLTAADTATYFCARSGSVFGVVSLWGPGTLVTVSSGQPKAPSVFPLAPCCGDTPSSTVTLGCLVKGYLPEPVTVTWNSGTLTNGVRTFPSVRQSSGLYSLSSVVSVTSSSQPVTCNVAHPATNTKVDKTVAPSTCSGTKHHHHHH |
Light Chain: L
Mutation: NULL
| >7NRH_L|Chain B[auth L]|Fab fragment HTN-Gn1 Light chain|Oryctolagus cuniculus (9986) TGQVLTQTPASVSEPVEGTVTIKCQASQSINNWLSWYQQRPGQPPKLLIYDASTVASGVSSRFKGSGSGTEFTLTISDLECADAATYACQSYGYGISITDNSAFGGGTEVVVRGDPVAPSVLIFPPAADQVATGTVTIVCVANKYFPDVTVTWEVDGTTQTTGIENSKTPQNSADCTYNLSSTLTLTSTQYNSHKEYTCKVTQGTTSVVQSFNRGDC |
Antigen
Chain: A
Mutation: NULL
| >7NRH_A|Chain C[auth A]|Envelope polyprotein|Hantaan orthohantavirus (3052480) TGSLRNVYDMKIECPHTVSFGENSVIGYVELPPMPLADTAQMVPESSCSMDNHQSINTITKYTQVIWRGKADPGQSSQNSFETVSTEVDLKGTCVLKHKMVEESYRSRKSITCYDLSCNSTFCKPTLYMIVPIHACNMMKSCLIALGPYRVQVVYERTYCMTGVLIEGKCFVPDQSVVSIIKHGIFDIASVHVVCFFVAVKGNTYKLFEQVKKSFESTCNDTENKVQGYYICIVGGNSAPIYVPTLDDFRSMEAFTGIFKSPHGEDHDLAGEEIASYSIVGPANAKVPHSASSDTLSLIAYSGIPSYSSLSILTSSTDAKHVFSPGLFPKLNHTNCDKSAIPLTWTGMIDLPGYYEGTKHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition H: GLN3 PHE28 SER31 SER32 THR33 HIS34 TRP35 TYR54 ASN57 THR58 ASP60 TYR62 ARG100 GLY102 SER103 VAL104 VAL107 SER109 L: ASN31 TRP33 LEU47 TYR50 ASP51 ALA56 SER57 TYR94 GLY95 ILE96 ILE98 THR99 A: PRO50 LEU51 ALA52 THR54 ALA55 GLN79 ILE81 TRP82 ARG83 GLY84 LYS85 ALA86 ASP87 PRO88 GLY89 SER91 SER92 ASN94 PHE96 GLU97 VAL99 THR101 GLU102 VAL103 LEU105 ASN134 SER135 THR136 PHE137 SER291 SER293 VAL295 SER317 GLY318 ILE319 PHE338 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
