Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4474 |
| PDBID: | 7NQK |
| Chains: | B_A |
| Organism: | Rattus norvegicus, Lama glama |
| Method: | EM |
| Resolution (Å): | 3.50 |
| Reference: | 10.1126/sciadv.abh3355 |
| Antibody | |
| Antibody: | D8 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Solute carrier family 15 member 2 |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: B
Mutation: NULL
| >7NQK_B|Chain B|nanobody|Lama glama (9844) GPSQVQLVESGGGLVQPGGSLRLLCVASGRPFNDYDMGWFRQAPGKEREFVASISWSGRVTDYSDSMKGRCTVSRDNAKGTMFLQMSNLVPRDTAVYYCAAARRRWTFKATNTEEFYETWGQGTQVTVSSA |
Antigen
Chain: A
Mutation: NULL
| >7NQK_A|Chain A|Solute carrier family 15 member 2|Rattus norvegicus (10116) MNPFQKNESKETLFSPVSTEEMLPRPPSPPKKSPPKIFGSSYPVSIAFIVVNEFCERFSYYGMKAVLTLYFLYFLHWNEDTSTSVYHAFSSLCYFTPILGAAIADSWLGKFKTIIYLSLVYVLGHVFKSLGAIPILGGKMLHTILSLVGLSLIALGTGGIKPCVAAFGGDQFEEEHAEARTRYFSVFYLAINAGSLISTFITPMLRGDVKCFGQDCYALAFGVPGLLMVLALVVFAMGSKMYRKPPPEGNIVAQVIKCIWFALCNRFRNRSGDLPKRQHWLDWAAEKYPKHLIADVKALTRVLFLYIPLPMFWALLDQQGSRWTLQANKMNGDLGFFVLQPDQMQVLNPFLVLIFIPLFDLVIYRLISKCRINFSSLRKMAVGMILACLAFAVAALVETKINGMIHPQPASQEIFLQVLNLADGDVKVTVLGSRNNSLLVESVSSFQNTTHYSKLHLEAKSQDLHFHLKYNSLSVHNDHSVEEKNCYQLLIHQDGESISSMLVKDTGIKPANGMAAIRFINTLHKDLNISLDTDAPLSVGKDYGVSAYRTVLRGKYPAVHCETEDKVFSLDLGQLDFGTTYLFVITNITSQGLQAWKAEDIPVNKLSIAWQLPQYVLVTAAEVMFSVTGLEFSYSQAPSSMKSVLQAAWLLTVAVGNIIVLVVAQFSGLAQWAEFVLFSCLLLVVCLIFSVMAYYYVPLKSEDTREATDKQIPAVQGNMINLETKNTRLVEGENLYFQ |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition B: GLN13 SER17 LEU18 ARG19 ASP33 SER52 TRP53 ARG56 VAL57 THR58 ASP59 THR69 ASP73 ALA75 LYS76 PHE80 GLN82 ARG101 ARG102 TRP103 THR104 PHE105 LYS106 ALA107 THR108 ASN109 THR110 GLU112 PHE113 A: LEU72 TYR73 LEU75 HIS76 TRP77 ASN78 GLU79 ASP80 THR81 SER84 VAL85 LYS139 THR143 LEU147 SER530 THR533 ASP534 PRO557 ALA558 VAL559 HIS560 LYS566 VAL567 PHE568 SER569 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
