Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4465 |
| PDBID: | 7NMU |
| Chains: | D_A |
| Organism: | Homo sapiens, Lama glama |
| Method: | XRD |
| Resolution (Å): | 2.50 |
| Reference: | 10.1182/blood.2020009440 |
| Antibody | |
| Antibody: | 7NMU_2 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | Platelet glycoprotein VI |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: D
Mutation: NULL
| >7NMU_D|Chain C[auth CCC], D[auth DDD]|Nanobody 2|Lama glama (9844) QVQLQESGGGLVQPGGSLRLSCAAAGFTFDYYAIAWFRQAPGKEREGVSCISSSDGTTYYADSVKGRFTISKDNAKNTMYLQMNSLKPEDTAVYYCATSPLYSTNDRCISEDYDYWGQGTQVTVSSLVPR |
Antigen
Chain: A
Mutation: NULL
| >7NMU_A|Chain A[auth AAA], B[auth BBB]|Platelet glycoprotein VI|Homo sapiens (9606) DKLASSGPLPKPSLQALPSSLVPLEKPVTLRCQGPPGVDLYRLEKLSSSRYQDQAVLFIPAMKRSLAGRYRCSYQQGSLWSLPSDQLELVATGVFAKPSLSAQPGPAVSSGGDVTLQCQTRYGFDQFALYKEGDPAPYKNPERWYRASFPIITVTAAHSGTYRCYSFSSRDPYLWSAPSDPLELVVTGDPIEGR |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition D: GLN1 VAL2 GLY26 PHE27 TYR31 TYR32 SER53 SER54 SER99 PRO100 LEU101 TYR102 SER103 THR104 ASN105 GLU111 ASP112 TYR113 ASP114 TYR115 A: GLU25 LYS26 PRO27 ARG42 LYS45 SER49 ARG50 TYR51 GLN52 ASP53 GLN54 LEU57 PHE58 ILE59 PRO60 ALA61 LYS63 SER65 LEU66 TYR70 THR187 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
