Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4397 |
| PDBID: | 7N0V |
| Chains: | HL_C |
| Organism: | Mus musculus, Betula pendula |
| Method: | XRD |
| Resolution (Å): | 3.71 |
| Reference: | 10.1016/j.jaci.2021.05.038 |
| Antibody | |
| Antibody: | REGN5715 Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | Atisnolerbart(Phase-III) |
| Antigen | |
| Antigen: | Major pollen allergen Bet v 1-A |
| Antigen mutation: | Yes |
| Durg Target: | |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >7N0V_H|Chain A[auth H]|REGN5715 Fab heavy chain|Mus musculus (10090) QVQLVQSGAEVKKPGASVKVSCKASGYTFISYNIFWVRQATGQGLDWMGWMNPFRNNAGYAQKFQGRVTVTWDTSISTAYMELSSLSSEDTAIYYCAREHGSSWGFFDYWGQGTLVTVSSASTKGPSVFPLAPCSRSTSESTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTKTYTCNVDHKPSNTKVDKRVESKYGPP |
Light Chain: L
Mutation: NULL
| >7N0V_L|Chain B[auth L]|REGN5715 Fab light chain|Mus musculus (10090) EIVLTQSPGTLSLSPGERATLSCRASQSVSSSYLAWYQQKPGQAPRLLIYGASSRATGIPDRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYGSSPWTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC |
Antigen
Chain: C
Mutation: F62L
| >7N0V_C|Chain C|Major pollen allergen Bet v 1-A|Betula pendula (3505) GVFNYETETTSVIPAARLFKAFILDGDNLFPKVAPQAISSVENIEGNGGPGTIKKISFPEGLPFKYVKDRVDEVDHTNFKYNYSVIEGGPIGDTLEKISNEIKIVATPDGGSILKISNKYHTKGDHEVKAEQVKASKEMGETLLRAVESYLLAHSDAYNEQKLISEEDLGGEQKLISEEDLHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition H: THR28 ILE30 SER31 TYR32 ASN33 ASN52 PHE54 ARG55 ARG98 HIS100 GLY101 SER102 SER103 TRP104 L: TYR33 C: LEU24 ASP25 GLU42 ASN43 ILE44 GLU45 GLY46 ASN47 GLY48 GLY49 PRO50 GLY51 THR52 LYS54 VAL74 HIS76 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
