Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 4391 |
| PDBID: | 7N0I |
| Chains: | J_C |
| Organism: | Severe acute respiratory syndrome coronavirus 2, Lama glama |
| Method: | XRD |
| Resolution (Å): | 2.20 |
| Reference: | 10.3389/fimmu.2021.719037 |
| Antibody | |
| Antibody: | E2 Nanobody |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | SARS-CoV-2 Spike glycoprotein CTD |
| Antigen mutation: | No |
| Durg Target: | |
Sequence information
Antibody
Chain: J
Mutation: NULL
| >7N0I_J|Chain H[auth I], I[auth J], J[auth K], K[auth L]|Single-domain antibody E2|Lama glama (9844) MAEVQLQASGGGLVQAGGSLRLSCAASGRTDSTQHMAWFRQAPGKEREFVTAIQWRGGGTSYTDSVKGRFTISRDNAKNTVYLEMNSLKPEDTAVYYCATNTRWTYFSPTVPDRYDYWGQGTQVTVSSAAALEHHHHHH |
Antigen
Chain: C
Mutation: NULL
| >7N0I_C|Chain A, B, C, D, E, F, G, L[auth H]|Nucleoprotein|Severe acute respiratory syndrome coronavirus 2 (2697049) NVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFP |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| Chain residues position delta_SASA
: residuesposition J: GLN52 GLY55 GLY56 GLY57 THR58 SER59 TYR60 LYS65 TRP102 THR103 TYR104 PHE105 SER106 C: PRO279 GLU280 GLN281 THR282 GLN283 ASN285 ARG319 ILE320 GLY321 GLU323 THR325 SER327 TRP330 THR332 TYR333 THR334 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 6 Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 6 Å from any atom.
Interacting residues (Atom distance based)
