Details
Structure visualisation
Entry information
| Complex | |
| AACDB_ID: | 439 |
| PDBID: | 3C09 |
| Chains: | HL_D |
| Organism: | Mus musculus, Homo sapiens |
| Method: | XRD |
| Resolution (Å): | 3.20 |
| Reference: | 10.1016/j.ccr.2008.02.019 |
| Antibody | |
| Antibody: | matuzumab Fab |
| Antibody mutation: | No |
| INN (Clinical Trial): | |
| Antigen | |
| Antigen: | domain III of the extracellular region of Epidermal growth factor receptor |
| Antigen mutation: | No |
| Durg Target: | Q504U8; P00533; |
Sequence information
Antibody
Heavy Chain: H
Mutation: NULL
| >3C09_H|Chain B[auth C], E[auth H]|Matuzumab Fab Heavy chain|Mus musculus (10090) QVQLVQSGAEVKKPGASVKVSCKASGYTFTSHWMHWVRQAPGQGLEWIGEFNPSNGRTNYNEKFKSKATMTVDTSTNTAYMELSSLRSEDTAVYYCASRDYDYAGRYFDYWGQGTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKS |
Light Chain: L
Mutation: NULL
| >3C09_L|Chain A[auth B], D[auth L]|Matuzumab Fab Light chain|Mus musculus (10090) DIQMTQSPSSLSASVGDRVTITCSASSSVTYMYWYQQKPGKAPKLLIYDTSNLASGVPSRFSGSGSGTDYTFTISSLQPEDIATYYCQQWSSHIFTFGQGTKVEIKRTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGE |
Antigen
Chain: D
Mutation: NULL
| >3C09_D|Chain C[auth A], F[auth D]|Epidermal growth factor receptor|Homo sapiens (9606) LEEKKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKHHHHHH |
Interaction
1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.
Interacting residues (ΔSASA based)
| L: TYR31 ASP49 TRP90 HIS93 PHE95 H: THR30 SER31 HIS32 TRP33 HIS35 GLU50 ASN52 SER54 ASN55 ARG57 ASN59 ARG99 ASP100 TYR101 ASP102 TYR103 GLY105 TYR107 D: LYS430 GLU431 SER433 ASP434 ASP436 ASN449 ILE451 ASN452 TRP453 LYS454 LYS455 LEU456 PHE457 GLY458 THR459 SER460 GLY461 GLN462 LYS463 THR464 ILE466 |
2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.
Interacting residues (Atom distance based)
