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Entry information

Complex
AACDB_ID: 438
PDBID: 3BT2
Chains: HL_U
Organism: Homo sapiens, Mus musculus
Method: XRD
Resolution (Å): 2.50
Reference: 10.1038/nsmb.1404
Antibody
Antibody: 3BT2 Fab
Antibody mutation: No
INN (Clinical Trial):
Antigen
Antigen: Urokinase plasminogen activator surface receptor
Antigen mutation: No
Durg Target: Q03405

Sequence information

Antibody

Heavy Chain: H
Mutation: NULL

>3BT2_H|Chain D[auth H]|anti-uPAR antibody, heavy chain|Mus musculus (10090)
GVKLQQSGPEVVKPGASVKISCKASGYSFTNFYIHWVKQRPGQGLEWIGWIFHGSDNTEYNEKFKDKATLTADTSSSTAYMQLSSLTSEDSAVYFCARWGPHWYFDVWGQGTTVTVSSAKTTPPSVYPLAPGNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTLSSSVTVPSSTWPSETVTCNVAHPASSTKVDKKIAAAG

Light Chain: L
Mutation: NULL

>3BT2_L|Chain C[auth L]|anti-uPAR antibody, light chain|Mus musculus (10090)
DIVLTQSPDITAASLGQKVTITCSASSSVSYMHWYQQKSGTSPKPWIFEISKLASGVPARFSGSGSGTSYSLTISSMEAEDAAIYYCQQWNYPFTFGGGTKLEIKRADAAPTVSIFPPSSEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTLTKDEYERHNSYTCEATHKTSTSPIVKSFNRNEC

Antigen

Chain: U
Mutation: NULL

>3BT2_U|Chain E[auth U]|Urokinase plasminogen activator surface receptor|Homo sapiens (9606)
RSLRCMQCKTNGDCRVEECALGQDLCRTTIVRLWEEGEELELVEKSCTHSEKTNRTLSYRTGLKITSLTEVVCGLDLCNQGNSGRAVTYSRSRYLECISCGSSDMSCERGRHQSLQCRSPEEQCLDVVTHWIQEGEEGRPKDDRHLRGCGYLPGCPGSNGFHNNDTFHFLKCCNTTKCNEGPILELENLPQNGRQCYSCKGNSTHGCSSEETFLIDCRGPMNQCLVATGTHEPKNQSYMVRGCATASMCQHAHLGDAFSMNHIDVSCCTKSGCNHPDLDVQYR

Interaction

1、Solvent accessible surface areas (SASA) were calculated (Naccess V2.1.1) for each residue in antibody and antigen, respectively. The residues with SASA loss in binding of more than 1Å2 were classified as interacting residues.

Interacting residues (ΔSASA based)

L: SER31 TYR32 TRP91 ASN92 TYR93 PHE96

H: TYR33 TRP50 HIS52 ASP55 ASN56 THR57 GLU58 TRP95 HIS98 TRP99

U: PRO180 GLU185 ASN186 LEU187 PRO188 GLN189 ASN190 GLY191 ARG192 ARG216 GLY217 PRO218 ASN220 GLN221 ALA244 THR267 LYS268 SER269

2、We defined interacting paratope-epitope residues by a distance cutoff of < 5Å . Two amino acids are considered as interacting residues if they have at least one atom within a distance of 5 Å from any atom.

Interacting residues (Atom distance based)

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